AG06095
Epithelial from Retina of eye
Description:
SV40 VIRUS-TRANSFORMED APPARENTLY HEALTHY: FETAL TISSUE
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Retina of eye
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Cell Type
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Epithelial
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Tissue Type
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Retina, pigmented
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Transformant
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Simian Virus 40
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Sample Source
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Epithelial from Retina of eye
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Permanent line RPE 18 SV2 was obtained by transformation of a fetus-derived retinal pigmented epithelial cell line with SV-40, strain Rh 911 virus. This transformed line appears stable without a crisis phase. It is T antigen positive and does not pigment. The morphology is epithelial-like. |
| Obin M, Shang F, Gong X, Handelman G, Blumberg J, Taylor A, Redox regulation of ubiquitin-conjugating enzymes: mechanistic insights using the thiol-specific oxidant diamide. FASEB J12:561-9 1998 |
| PubMed ID: 9576483 |
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| Jahngen-Hodge J, Obin MS, Gong X, Shang F, Nowell TR Jr, Gong J, Abasi H, Blumberg J, Taylor A, Regulation of ubiquitin-conjugating enzymes by glutathione following oxidative stress. J Biol Chem272:28218-26 1997 |
| PubMed ID: 9353272 |
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| Gupta RS, Dudani AK, Species-specific differences in the toxicity of rhodamine 123 towards cultured mammalian cells. J Cell Physiol130:321-7 1987 |
| PubMed ID: 3558490 |
| Split Ratio |
1:18 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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