AG06096
Epithelial from Retina of eye
Description:
SV40 VIRUS-TRANSFORMED APPARENTLY HEALTHY: FETAL TISSUE
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Retina of eye
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Cell Type
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Epithelial
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Tissue Type
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Retina, pigmented
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Transformant
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Simian Virus 40
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Sample Source
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Epithelial from Retina of eye
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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45,X,der(7)(pter>q22::q11.2>q31.1:),-22,+mar[46]/46,X,der(7)(pter>q22::q11.2>q31.1:),-22,+mar,+mar[4]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Permanent line RPE 28 SV4 was obtained by transformation of a fetus-derived retinal pigmented epithelial cell line with SV-40, strain Rh 911 virus. This transformed line appears stable without a crisis phase. It is T antigen positive and does not pigment. The morphology is epithelial-like. An untransformed cell line from this same donor is AG05337A. |
| Oshiro N, Pajor AM, Functional characterization of high-affinity Na(+)/dicarboxylate cotransporter found in Xenopus laevis kidney and heart American journal of physiology Cell physiology289:C1159-68 2005 |
| PubMed ID: 15944208 |
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| Pajor AM, Randolph KM, Conformationally sensitive residues in extracellular loop 5 of the Na+/dicarboxylate co-transporter The Journal of biological chemistry280:18728-35 2005 |
| PubMed ID: 15774465 |
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| Weng TX, Godley BF, Jin GF, Mangini NJ, Kennedy BG, Yu AS, Wills NK, Oxidant and antioxidant modulation of chloride channels expressed in human retinal pigment epithelium. Am J Physiol Cell Physiol283(3):C839-49 2002 |
| PubMed ID: 12176741 |
| Split Ratio |
1:10 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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