AG06242
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
GRC |
Biopsy Source
|
Arm
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Arm
|
Race
|
White
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
46,XY/46,XY,t(2;15)(2pter>2q33::15p11.1> 15pter;15qter>15p11.1::2q33>2qter)
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Senescence |
33 |
PDL at Freeze |
12 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XY/46,XY,t(2;15)(2pter>2q33::15p11.1>15pter;15qter> 15p11.1::2q33>2qter); balanced; 94%/6%. A culture initiated from a biopsy taken 7 years later from this same donor is AG11648. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
PDL at Senescence |
33 |
Cumulative PDL at Freeze |
12 |
Split Ratio |
1:4 |
Temperature |
37 C |
Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|