AG08033
Endothelial from Aorta, thoracic
Description:
EQUUS
ORDER: PERISSODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Equus caballus
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Common Name
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horse
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Remarks
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| PDL at Senescence |
45 |
| PDL at Freeze |
31 |
| Passage Frozen |
4 |
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| Remarks |
Standardbred horse. This line is a second cloned culture from the same population of cells as AG8033. The thoracic aortawas removed post-mortem on 2/26/85. The primary culture was initiated using cells released from the lumen of the vessel by collagenase digestion. The culture was cloned at PDL 5, expanded and frozen. The cell morphology is polygonal. Culture submitted by F Angelosanto (#0477). The culture was grown on gelatincoated flasks and the medium was supplemented with ECGS (0.02 mg/ml) and heparin (0.05 mg/ml). Culture medium: AMMC 20U (with supplements). This cell line will only be shipped frozen. |
| PDL at Senescence |
45 |
| Cumulative PDL at Freeze |
31 |
| Passage Frozen |
4 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides/MCDB110, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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