AG08592
Endothelial from Aorta, thoracic
Description:
BOS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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60,XY
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| PDL at Freeze |
15 |
| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Genbank |
COX1 |
| Remarks |
Angus breed. The age of the donor is estimated. The thoracic aorta was removed immediately post-mortem on 12/3/85. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. The cell morphology is polygonal. The karyotype is 60,XY; normal diploid male. The cells were grown on gelatin-coated flasks in medium supplemented with ECGS (0.02 mg/ml) and heparin (0.05 mg/ml). The culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). Culture medium: HF12 15U with supplements. For lifespan determination this cell line was cultured in Ham's F12 medium with 10% inactivated fetal bovine serum. A smooth muscle cell culture from this calf is AG08593A. This cell line will only be shipped frozen. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Foresti R, Bains S, Sulc F, Farmer PJ, Green CJ, Motterlini R, The interaction of nitric oxide with distinct hemoglobins differentially amplifies endothelial heme uptake and heme oxygenase-1 expression The Journal of pharmacology and experimental therapeutics317:1125-33 2006 |
| PubMed ID: 16495208 |
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| Deshpande RG, Khan MB, Genco CA, Invasion of aortic and heart endothelial cells by Porphyromonas gingivalis. Infect Immun66:5337-43 1998 |
| PubMed ID: 9784541 |
| Cumulative PDL at Freeze |
15 |
| Passage Frozen |
4 |
| Split Ratio |
1:7 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides/MCDB110, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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