AG08595
Smooth muscle from Aorta, thoracic
Description:
BOS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Smooth muscle
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Smooth muscle from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| PDL at Senescence |
85 |
| PDL at Freeze |
7 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase and Glucose-6-Phosphate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Angus breed. The age of the donor is estimated. The thoracic aorta was removed immediately post-mortem on 12/3/85. Strips of the medial layer of the vessel were removed, minced, and used as explants to initiate the culture. The cell morphology is fibroblast-like. The culture tested positive for alpha-smooth muscle actin and negative for the endothelial cell-specific von Willebrand factor (immunofluorescence assays). For lifespan determination this cell line was cultured in Ham's F12 medium with 10% inactivated fetal bovine serum. An endothelial cell culture from this cow is AG08594. |
| Motterlini R, Clark JE, Foresti R, Sarathchandra P, Mann BE, Green CJ, Carbon monoxide-releasing molecules: characterization of biochemical and vascular activities. Circ Res90(2):E17-24 2002 |
| PubMed ID: 11834719 |
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| Panetti TS, Nowlen J, Mosher DF, Sphingosine-1-phosphate and lysophosphatidic acid stimulate endothelial cell migration. Arterioscler Thromb Vasc Biol20(4):1013-9 2000 |
| PubMed ID: 10764666 |
| PDL at Senescence |
85 |
| Cumulative PDL at Freeze |
7 |
| Passage Frozen |
2 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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