AG08733
Smooth muscle from Aorta, thoracic
Description:
BOS
ORDER: CETARTIODACTYLA
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Animals |
|
Biopsy Source
|
Aorta, thoracic
|
|
Cell Type
|
Smooth muscle
|
|
Tissue Type
|
Artery
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Smooth muscle from Aorta, thoracic
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Bos taurus
|
|
Common Name
|
cow
|
|
Remarks
|
|
| PDL at Senescence |
48 |
| PDL at Freeze |
5 |
| Passage Frozen |
4 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase and Glucose-6-Phosphate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Holstein breed. The thoracic aorta was removed 15 minutes post-mortem on 3/11/86. Strips of the medial layer were removed, minced, and used as explants to initiate the culture. Cell morphology is fibroblast-like. The culture tested positive for alpha-smooth muscle actin and negative for the endothelial cell-specific von Willebrand factor (immunofluorescence assays). For lifespan determination this cell line was cultured in Ham's F12 medium with 15% inactivated fetal bovine serum. |
| Motterlini R, Clark JE, Foresti R, Sarathchandra P, Mann BE, Green CJ, Carbon monoxide-releasing molecules: characterization of biochemical and vascular activities. Circ Res90(2):E17-24 2002 |
| PubMed ID: 11834719 |
| |
| Panetti TS, Nowlen J, Mosher DF, Sphingosine-1-phosphate and lysophosphatidic acid stimulate endothelial cell migration. Arterioscler Thromb Vasc Biol20(4):1013-9 2000 |
| PubMed ID: 10764666 |
| PDL at Senescence |
48 |
| Cumulative PDL at Freeze |
5 |
| Passage Frozen |
4 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|