AG09602
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5.48 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remark |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like and the karyotype is 46,XX with 6% of the cells examined showing random chromosome loss. A culture initiated from a biopsy taken 5 years earlier from this same donor is AG05247E. |
| Park JW, Han SB, Hah J, Lee G, Kim JK, Kim SH, Kim DH, Biological Aging Modulates Cell Migration via Lamin A/C-Dependent Nuclear Motion Micromachines11: 2020 |
| PubMed ID: 32847135 |
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| Larrieu D1, Viré E2, Robson S2, Breusegem SY2, Kouzarides T2, Jackson SP1., Inhibition of the acetyltransferase NAT10 normalizes progeric and aging cells by rebalancing the Transportin-1 nuclear import pathway Science Signaling11: 2018 |
| PubMed ID: 29970603 |
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| Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19: 2018 |
| PubMed ID: 30567591 |
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| Cao K, Blair CD, Faddah DA, Kieckhaefer JE, Olive M, Erdos MR, Nabel EG, Collins FS, Progerin and telomere dysfunction collaborate to trigger cellular senescence in normal human fibroblasts The Journal of clinical investigation19: 2010 |
| PubMed ID: 21670498 |
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| Goldman RD, Shumaker DK, Erdos MR, Eriksson M, Goldman AE, Gordon LB, Gruenbaum Y, Khuon S, Mendez M, Varga R, Collins FS, Accumulation of mutant lamin A causes progressive changes in nuclear architecture in Hutchinson-Gilford progeria syndrome. Proc Natl Acad Sci U S A101(24):8963-8 2004 |
| PubMed ID: 15184648 |
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| Cristofalo VJ, Allen RG, Pignolo RJ, Martin BG, Beck JC, Relationship between donor age and the replicative lifespan of human cells in culture: a reevaluation. Proc Natl Acad Sci U S A95:10614-9 1998 |
| PubMed ID: 9724752 |
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| Allen RG, Keogh BP, Tresini M, Gerhard GS, Volker C, Pignolo RJ, Horton J, Cristofalo VJ, Development and age-associated differences in electron transport potential and consequences for oxidant generation. J Biol Chem272:24805-12 1997 |
| PubMed ID: 9312077 |
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| Allen RG, Keogh BP, Gerhard GS, Pignolo R, Horton J, Cristofalo VJ, Expression and regulation of superoxide dismutase activity in human skin fibroblasts from donors of different ages. J Cell Physiol165:576-87 1995 |
| PubMed ID: 7593237 |
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| Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, Medrano EE, Linskens M, Rubelj I, Pereira-Smith O, et al, A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci U S A92:9363-7 1995 |
| PubMed ID: 7568133 |
| Cumulative PDL at Freeze |
5.48 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
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