AG09897
Endothelial from Vein, iliac
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Vein, iliac
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Cell Type
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Endothelial
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Tissue Type
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Vein
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Transformant
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Untransformed
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Sample Source
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Endothelial from Vein, iliac
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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87~92,XXX~XXXX,-7,-11[cp9]/46,XX[1]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
22 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line HIVE-98/7-1. The iliac vein was removed from a braindead, heartbeating cadaver, renal donor. The donor died of a subarachnoid hemorrhage. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. Cell morphology is polygonal. The karyotype is 46,XX/2n+/3n+-. The culture was grown on gelatin-coated flasks and the medium was supplemented with 0.02 mg/ml ECGS and 0.05 mg/ml heparin.Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Chang E, Harley CB, Telomere length and replicative aging in human vascular tissues. Proc Natl Acad Sci U S A92:11190-4 1995 |
| PubMed ID: 7479963 |
| Cumulative PDL at Freeze |
22 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Medium 199 in Earl BSS with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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