AG10427
Endothelial from Artery, iliac
Description:
SV40 VIRUS-TRANSFORMED APPARENTLY HEALTHY CULTURE
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Apparently Healthy Collection |
|
Biopsy Source
|
Artery, iliac
|
|
Cell Type
|
Endothelial
|
|
Tissue Type
|
Artery
|
|
Transformant
|
Simian Virus 40
|
|
Sample Source
|
Endothelial from Artery, iliac
|
|
Race
|
Black/African American
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
57~77,XXYY~XYY~XXY,+2,+3,-3,-4,+5,+6,+7,der(9)t(9;?)(q13;?),der(9)t(9;?)(q21;?),-10,-10,del(10)(p12),-11,-12,-13,-13,+14,-15,-15,-16,der(17)t(15;17)(q13;p13),+der(17)t(15;17),+18,-19,+der(20)t(20;?)(q12;?),-20,+21,-21,+22,-22,+2~8 markers [cp10]
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
The culture is a post-crisis SV40 virus-transformed derivative of line HIAE 43/7-13 (AG09872A). It was derived on 2/15/88 by infecting human iliac artery endothelial line AG09872A at PDL 15 with SV40 virus (strain Rh 911). Foci of transformed cells appeared approximately 18 days postinfection and crisis occurred 27 PDLs post-infection. T-antigen positive cells are present and the culture tested negative for von Willebrand factor after crisis. |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|