AG10671
Smooth muscle from Artery, carotid
Description:
BOS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Artery, carotid
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Cell Type
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Smooth muscle
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Smooth muscle from Artery, carotid
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| PDL at Senescence |
35 |
| PDL at Freeze |
14 |
| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Holstein breed. The carotid artery was removed immediately post-mortem from an adult of unknown age on 4/17/87. The culture was initiated 4 hours later using cells released from the lumen of the vessel by collagenase/ elastase digestion. The cell morphology is fibroblast-like. The culture tested positive for alpha-smooth muscle actin (immunofluorescence assay). Culture grows slowly. |
| PDL at Senescence |
35 |
| Cumulative PDL at Freeze |
14 |
| Passage Frozen |
5 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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