AG10697
Endothelial from Artery, pulmonary
Description:
BOS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Artery, pulmonary
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Artery, pulmonary
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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58-65,XY
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| PDL at Senescence |
53 |
| PDL at Freeze |
14 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Holstein breed. The pulmonary artery was taken 30 minutes post-mortem on 6/07/89. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. The cell morphology is polygonal. This culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). The karyotype is 58-65,XY with random chromosome losses, gains, and marker chromosomes in each cell studied. Other cultures from this calf are AG10555 and AG10605. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Ziegelstein RC, Corda S, Pili R, Passaniti A, Lefer D, Zweier JL, Fraticelli A, Capogrossi MC, Initial contact and subsequent adhesion of human neutrophils or monocytes to human aortic endothelial cells releases an endothelial intracellular calcium store. Circulation90(4):1899-907 1994 |
| PubMed ID: 7923678 |
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| Pili R, Corda S, Passaniti A, Ziegelstein RC, Heldman AW, Capogrossi MC, Endothelial cell Ca2+ increases upon tumor cell contact and modulates cell-cell adhesion. J Clin Invest92(6):3017-22 1993 |
| PubMed ID: 8254056 |
| PDL at Senescence |
53 |
| Cumulative PDL at Freeze |
14 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Heat Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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