AG10767
Endothelial from Artery, iliac
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Artery, iliac
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Artery, iliac
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
9 |
| Passage Frozen |
8 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line HIAE-109. The iliac artery was removed from a braindead, heartbeating cadaver, organ donor on 5/26/88. The cause of death was unknown. The culture was initiated on 5/26/88 using cells released from the lumen of the vessel by collagenase digestion. Cell morphology is polygonal. Most cells were positive by immunofluorescence assay for endothelial cell-specific von Willebrand factor. Some cells were positive for alpha-smooth muscle actin (2% at PDL 16 and 8% at PDL 31). The culture was grown on gelatin-coated flasks and the medium was supplemented with 0.02 mg/ml ECGS and 0.05 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. An iliac vein endothelial cell culture from this same donor is AG10768. |
| Chang E, Harley CB, Telomere length and replicative aging in human vascular tissues. Proc Natl Acad Sci U S A92:11190-4 1995 |
| PubMed ID: 7479963 |
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| Hauser IA, Johnson DR, Madri JA, Differential induction of VCAM-1 on human iliac venous and arterial endothelial cells and its role in adhesion. J Immunol151(10):5172-85 1993 |
| PubMed ID: 7693806 |
| Cumulative PDL at Freeze |
9 |
| Passage Frozen |
8 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Medium 199 in Earl BSS with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Heat Inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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