AG10768
Endothelial from Vein, iliac
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Vein, iliac
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Cell Type
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Endothelial
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Tissue Type
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Vein
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Transformant
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Untransformed
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Sample Source
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Endothelial from Vein, iliac
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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47,XX,add(1)(q44),t(3;16)(p13;q22),+11[24]/46,XX,dup(1)(q25q11)[12]/46,XX,-13,+mar[4]/91,XXXX,-13[4]/47,XX,+11[2]/92,XXXX[1]/46,XX[3]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
21 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line HIVE-109. The iliac vein was removed from a braindead, heartbeating cadaver, organ donor on 5/26/88. The cause of death was unknown. The culture was initiated on 5/27/88 using cells released from the lumen of the vessel by collagenase digestion. Cell morphology is polygonal. Most cells were positive by immunofluorescence assay for endothelial cellspecific von Willebrand factor. Some cells were positive for alpha-smooth muscle actin (4% at PDL 16 and 6% at PDL 27). The culture was grown on gelatin-coated flasks and the medium was supplemented with 0.02 mg/ml crude ECGS and 0.05 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. The karyotype is unstable. An iliac artery endothelial cell culture from this same donor is AG10767. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Hauser IA, Johnson DR, Madri JA, Differential induction of VCAM-1 on human iliac venous and arterial endothelial cells and its role in adhesion. J Immunol151(10):5172-85 1993 |
| PubMed ID: 7693806 |
| Cumulative PDL at Freeze |
21 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Medium 199 in Earl BSS with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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