AG10773
Endothelial from Vein, iliac
Description:
APPARENTLY HEALTHY INDIVIDUAL
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Apparently Healthy Collection |
|
Biopsy Source
|
Vein, iliac
|
|
Cell Type
|
Endothelial
|
|
Tissue Type
|
Vein
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Endothelial from Vein, iliac
|
|
Race
|
White
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Freeze |
20 |
| Passage Frozen |
8 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Line HIVE-86. The iliac vein was removed from a braindead, heartbeating cadaver, organ donor on 6/03/87. The donor died of a gun shot wound to the head and had no known diseases. The culture was initiated on 6/03/87 using cells released from the lumen of the vessel by collagenase digestion. Cell morphology is polygonal. The culture was grown on gelatin-coated flasks and the medium was supplemented with 0.02 mg/ml ECGS and 0.05 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. For lifespan determination this cell line was cultured in Ham's F12 medium and MCDB104 medium in a 1:1 mixture with 10% inactivated FBS and supplements (0.04-0.06mg/ml crude ECGF and 0.05mg/ml heparin). This culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). microgravity experiment in space (Mission STS-89/Mir). |
| Calzada MJ, Sipes JM, Krutzsch HC, Yurchenco PD, Annis DS, Mosher DF, Roberts DD, Recognition of the N-terminal modules of thrombospondin-1 and thrombospondin-2 by alpha6beta1 integrin. J Biol Chem278(42):40679-87 2003 |
| PubMed ID: 12909644 |
| Cumulative PDL at Freeze |
20 |
| Passage Frozen |
8 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Medium 199 in Earl BSS with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Heat Inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
|
|