AG11134
Epithelial from Breast organoid
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Breast organoid
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Cell Type
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Epithelial
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Tissue Type
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Breast
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Transformant
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Untransformed
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Sample Source
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Epithelial from Breast organoid
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5.19 |
| Passage Frozen |
15 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Tissue was obtained in 1980 during reduction mammoplasty; pathologic diagnosis was possible early minimal phase of fibrocystic disease. Mammary epithelial clumps or organoids were isolated by tissue dissection followed by partial enzymatic digestion with collagenase and hyaluronidase. Epithelial organoids were separated from the digested stromal elements by filtration and stored in liquid nitrogen. The culture was initiated 2/24/85 from thawed organoids by explantation in serum-free MCDB 170 plus supplements. Serial subcultivation resulted in the selection of a population of proliferative epithelial cells. Culture submitted as line 172R/AA765 at passage 6, PDL 25 (estimated by submitter), and has been frozen at the Repository at PDL 38. Culture is grown in serum-free medium and grows best with only 1 to 2% carbon dioxide. |
| Dietze EC, Bowie ML, Mrózek K, Caldwell LE, Neal C, Marjoram RJ, Troch MM, Bean GR, Yokoyama KK, Ibarra CA, Seewaldt VL, CREB-binding protein regulates apoptosis and growth of HMECs grown in reconstituted ECM via laminin-5 Journal of cell science118:5005-22 2005 |
| PubMed ID: 16219677 |
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| Kumaravel TS, Bristow RG, Detection of genetic instability at HER-2/neu and p53 loci in breast cancer cells sing Comet-FISH Breast cancer research and treatment91:89-93 2005 |
| PubMed ID: 15868435 |
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| Yee LD, Guo Y, Bradbury J, Suster S, Clinton SK, Seewaldt VL, The antiproliferative effects of PPARgamma ligands in normal human mammary epithelial cells Breast cancer research and treatment78:179-92 2003 |
| PubMed ID: 12725418 |
| |
| Mambo E, Nyaga SG, Bohr VA, Evans MK, Defective repair of 8-hydroxyguanine in mitochondria of MCF-7 and MDA-MB-468 human breast cancer cell lines. Cancer Res62(5):1349-55 2002 |
| PubMed ID: 11888904 |
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| Zhang Y, Rishi AK, Dawson MI, Tschang R, Farhana L, Boyanapalli M, Reichert U,
Shroot B, Van Buren EC, Fontana JA, S-phase arrest and apoptosis induced in normal mammary epithelial cells by a
novel retinoid. Cancer Res60(7):2025-32 2000 |
| PubMed ID: 10766194 |
| |
| Stampfer, Isolation and growth of human mammary epithelial cells. J Tissue Culture Methods9:107 (1985):2025-32 1985 |
| PubMed ID: 10766194 |
| Cumulative PDL at Freeze |
42.2 |
| Passage Frozen |
15 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEGM-mammary epithelial cell medium plus supplements |
| Serum |
none |
| Substrate |
Collagen IV-coated |
| Supplement |
Isoproterinol 10-5M |
| Supplement |
Transferrin 5ug/ml |
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