AG11138
Epithelial from Breast organoid
Description:
APPARENTLY HEALTHY INDIVIDUAL
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Apparently Healthy Collection |
|
Biopsy Source
|
Breast organoid
|
|
Cell Type
|
Epithelial
|
|
Tissue Type
|
Breast
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Epithelial from Breast organoid
|
|
Race
|
White
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Senescence |
53 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Non-tumor tissue with some cysts obtained in 1981 during mastectomy for infiltrating lobular carcinoma. Mammary epithelial clumps or organoids were isolated by tissue dissection followed by partial enzymatic digestion with collagenase and hyaluronidase. Epithelial organoids were separated from the digested stromal elements by filtration and stored in liquid nitrogen. The culture was initiated 5/84 from thawed organoids by explantation in serum-free MCDB 170 plus supplements. Serial subcultivation resulted in the selection of a population of proliferative epithelial cells. Culture submitted as line 192P/AA832 at passage 5, PDL 22 (estimated by submitter), and has been frozen at the Repository at PDL 34. Culture is grown in serum-free medium. |
| Stampfer, Isolation and growth of human mammary epithelial cells. J Tissue Culture Methods9:107 (1985): 1985 |
| PubMed ID: |
| PDL at Senescence |
53 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEGM-mammary epithelial cell medium plus supplements |
| Serum |
0% fetal bovine serum |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|