AG11184
Endothelial from Vein, iliac
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Vein, iliac
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Cell Type
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Endothelial
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Tissue Type
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Vein
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Transformant
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Untransformed
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Sample Source
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Endothelial from Vein, iliac
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
43 |
| PDL at Freeze |
26 |
| Passage Frozen |
11 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line HIVE-36. The iliac vein was removed from a braindead, heartbeating cadaver, organ donor on 9/28/84. Donor died of head trauma and had no known diseases. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. Cell morphology is polygonal. This culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). The culture was grown on gelatin-coated flasks and the medium was supplemented with 0.02 mg/ml ECGS and 0.05 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. Culture grows slowly. |
| Chang E, Harley CB, Telomere length and replicative aging in human vascular tissues. Proc Natl Acad Sci U S A92:11190-4 1995 |
| PubMed ID: 7479963 |
| PDL at Senescence |
43 |
| Cumulative PDL at Freeze |
26 |
| Passage Frozen |
11 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Medium 199 in Earl BSS with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Heat Inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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