AG11548
Smooth muscle from Artery, iliac
Description:
APPARENTLY HEALTHY INDIVIDUAL
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Apparently Healthy Collection |
|
Biopsy Source
|
Artery, iliac
|
|
Cell Type
|
Smooth muscle
|
|
Tissue Type
|
Artery
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Smooth muscle from Artery, iliac
|
|
Race
|
White
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Senescence |
36 |
| PDL at Freeze |
30 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Line HIAS-130T. The iliac artery was removed from a braindead, heartbeating cadaver, organ donor on 4/01/90. The donor died from a head injury and had no known diseases. The culture was initiated by explantation of tissue dissected from the medial layer. The culture tested positive for alphasmooth muscle actin (immunofluorescence assay). The culture was grown on gelatin-coated flasks, and the medium was supplemented with 0.02 mg/ml ECGS and 0.5 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. An iliac vein smooth muscle cell culture from this same donor is AG11549. |
| Mesri M, Wall NR, Li J, Kim RW, Altieri DC, Cancer gene therapy using a survivin mutant adenovirus. J Clin Invest108(7):981-90 2001 |
| PubMed ID: 11581299 |
| |
| Hauser IA, Renders L, Radeke HH, Sterzel RB, Goppelt-Struebe M, Mycophenolate mofetil inhibits rat and human mesangial cell proliferation by guanosine depletion. Nephrol Dial Transplant14(1):58-63 1999 |
| PubMed ID: 10052478 |
| PDL at Senescence |
36 |
| Cumulative PDL at Freeze |
30 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Medium 199 in Earl BSS with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
|
|