AG11645
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
GRC |
Biopsy Source
|
Arm
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Arm
|
Race
|
White
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
46,XY,inv(9)(p11q12)/46,XY,inv(9), t(8;10;14)(p21;p13;q24)
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Senescence |
25 |
PDL at Freeze |
7 |
Passage Frozen |
3 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
|
Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XY,inv(9)(p11q12)/46,XY,inv(9),t(8;10;14)(p21;p13;q24); balanced; 82%/18%. A culture initiated from a biopsy taken 4 years earlier from this same donor is AG09698A. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
PDL at Senescence |
25 |
Cumulative PDL at Freeze |
7 |
Passage Frozen |
3 |
Split Ratio |
1:3 |
Temperature |
37 C |
Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|