AG13177
Tumor-derived cell line from Embryonal carcinoma
Description:
MUS
ORDER: RODENTIA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Embryonal carcinoma
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Cell Type
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Tumor-derived cell line
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Tissue Type
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Tumor
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Transformant
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Untransformed
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Sample Source
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Tumor-derived cell line from Embryonal carcinoma
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Mouse embryonal carcinoma cell line B1(NG)C39D, a compaction-defective, 5bromodeoxyuridine-resistant, variant of line H6 selected for failure to compact as aggregates in culture and subsequently mutagenized with Nmethyl-N'-nitro-N-nitrosoguanidine. Aggregates of B1(NG)C39D fail to compact rapidly due to an unknown mutation since cells contain uvomoralin. The culture grows in thick patches. The same culture prior to chemical mutagenesis is AG13176. |
| Weng DE, Littlefield JW, Loss of expression of the uvomorulin gene in compaction-defective embryonal carcinoma cells. Somat Cell Mol Genet17:617-9 1991 |
| PubMed ID: 1767339 |
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| Littlefield JW, Whitehouse LL, Absence of uvomorulin in a slowly compacting variant of H6 embryonal carcinoma cells. Somat Cell Mol Genet16:191-4 1990 |
| PubMed ID: 2321096 |
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| Littlefield JW, Compaction-defective embryonal carcinoma cell variants. Dev Genet10:292-7 1989 |
| PubMed ID: 2791350 |
| Split Ratio |
1:25 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Minimum Essential Medium Modified for Suspension Cultures with 2mM L-glutamine or equivalent |
| Serum |
5% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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