AG13179
Tumor-derived cell line from Embryonal carcinoma
Description:
MUS
ORDER: RODENTIA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Embryonal carcinoma
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Cell Type
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Tumor-derived cell line
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Tissue Type
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Tumor
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Transformant
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Untransformed
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Sample Source
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Tumor-derived cell line from Embryonal carcinoma
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Genbank |
COX1 |
| Remarks |
Mouse embryonal carcinoma cell line 6B(NG)C25, a compaction-defective, 6thioguanine-resistant variant of line H6 selected for failure to compact as aggregates in culture and subsequently mutagenized with N-methyl-N'nitro-N-nitroso- guanidine. Aggregates of 6B(NG)C25 fail to compact rapidly due to a lack of uvomoralin caused by a deficiency of uvomoralin mRNA. The culture grows as a monolayer. The same culture prior to chemical mutagenesis is AG13178. |
| Weng DE, Littlefield JW, Loss of expression of the uvomorulin gene in compaction-defective embryonal carcinoma cells. Somat Cell Mol Genet17:617-9 1991 |
| PubMed ID: 1767339 |
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| Littlefield JW, Whitehouse LL, Absence of uvomorulin in a slowly compacting variant of H6 embryonal carcinoma cells. Somat Cell Mol Genet16:191-4 1990 |
| PubMed ID: 2321096 |
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| Littlefield JW, Compaction-defective embryonal carcinoma cell variants. Dev Genet10:292-7 1989 |
| PubMed ID: 2791350 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Minimum Essential Medium Modified for Suspension Cultures with 2mM L-glutamine or equivalent |
| Serum |
5% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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