AG13244
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
GRC |
|
Biopsy Source
|
Arm
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Arm
|
|
Race
|
White
|
|
Family Member
|
4
|
|
Relation to Proband
|
grandson
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
46,XY/46,XY,t(6;16)(p13;p21)
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Senescence |
28 |
| PDL at Freeze |
6 |
| Passage Frozen |
3 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XY/46,XY,t(6;16)(p13;p21); 96%/4% with 6% of the cells examined showing random chromosome loss/gain and 2% showing random chromosomal aberrations. The donor is the son of AG05807A, the grandson of AG04064, and the brother of AG05838B. A culture initiated from a biopsy taken 12 years earlier from this same donor is AG06962. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19: 2018 |
| PubMed ID: 30567591 |
| PDL at Senescence |
28 |
| Cumulative PDL at Freeze |
6 |
| Passage Frozen |
3 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|