AG13962
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Race
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White
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Family Member
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2
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Relation to Proband
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son
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY/46,XY,t(2;9)(q14;q21)/46,XY, t(1;4;5;14)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
25 |
| PDL at Freeze |
4 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The donor is obese. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XY/46,XY,t(2;9)(q14;q21)/46,XY, t(1;4;5;14); 74%/14%/12% with 2% of the cells showing random chromosome loss and 2% showing random chromosomal aberrations. A culture initiated from a biopsy taken 12 years earlier from this same donor is AG13105. Donor's father is AG05417. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19: 2018 |
| PubMed ID: 30567591 |
| PDL at Senescence |
25 |
| Cumulative PDL at Freeze |
4 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
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