AG13962
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Race
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White
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Family Member
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2
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Relation to Proband
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son
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY/46,XY,t(2;9)(q14;q21)/46,XY, t(1;4;5;14)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
25 |
| PDL at Freeze |
4 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remark |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The donor is obese. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XY/46,XY,t(2;9)(q14;q21)/46,XY, t(1;4;5;14); 74%/14%/12% with 2% of the cells showing random chromosome loss and 2% showing random chromosomal aberrations. A culture initiated from a biopsy taken 12 years earlier from this same donor is AG13105. Donor's father is AG05417. |
| Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19: 2018 |
| PubMed ID: 30567591 |
| PDL at Senescence |
25 |
| Cumulative PDL at Freeze |
4 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
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