AG14843
LCL from B-Lymphocyte
Description:
APPARENTLY HEALTHY INDIVIDUAL
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
"Intra-abdominal fat and risk of disease in children and adolescents" study
Apparently Healthy Collection |
|
Biopsy Source
|
Peripheral vein
|
|
Cell Type
|
B-Lymphocyte
|
|
Tissue Type
|
Blood
|
|
Transformant
|
Epstein-Barr Virus
|
|
Sample Source
|
LCL from B-Lymphocyte
|
|
Race
|
Black/African American
|
|
Ethnicity
|
AFRICAN-AMERICAN
|
|
Family Member
|
3
|
|
Relation to Proband
|
sister
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
46,XX
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Participant in the "intra-abdominal fat and risk of disease in children and adolescents" study. The culture was initiated on 11/26/97 by transformation of lymphocytes with Epstein Barr virus. The culture grows in suspension and the cell morphology is spherical. The karyotype is 46,XX with 4% of the cells examined showing random chromosome loss and 2% showing random chromosomal aberrations. Lymphoblast cultures from this donor's sisters are AG14841 and AG14842. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Goran MI, Metabolic precursors and effects of obesity in children: a decade of progress, 1990-1999. Am J Clin Nutr73(2):158-71 2001 |
| PubMed ID: 11157310 |
| |
| Goran MI, Bergman RN, Gower BA, Influence of total vs. visceral fat on insulin action and secretion in African American and white children. Obes Res9(8):423-31 2001 |
| PubMed ID: 11500522 |
| |
| Goran MI, Gower BA, Longitudinal study on pubertal insulin resistance. Diabetes50(11):2444-50 2001 |
| PubMed ID: 11679420 |
| |
| Herd SL, Gower BA, Dashti N, Goran MI, Body fat, fat distribution and serum lipids, lipoproteins and apolipoproteins in African-American and Caucasian-American prepubertal children. Int J Obes Relat Metab Disord25(2):198-204 2001 |
| PubMed ID: 11410820 |
| |
| Huang TT, Johnson MS, Figueroa-Colon R, Dwyer JH, Goran MI, Growth of visceral fat, subcutaneous abdominal fat, and total body fat in children. Obes Res9(5):283-9 2001 |
| PubMed ID: 11346669 |
| |
| Johnson MS, Figueroa-Colon R, Huang TT, Dwyer JH, Goran MI, Longitudinal changes in body fat in African American and Caucasian children: influence of fasting insulin and insulin sensitivity. J Clin Endocrinol Metab86(7):3182-7 2001 |
| PubMed ID: 11443186 |
| |
| Li C, Johnson MS, Goran MI, Effects of low birth weight on insulin resistance syndrome in caucasian and African-American children. Diabetes Care24(12):2035-42 2001 |
| PubMed ID: 11723079 |
| |
| Sun M, Gower BA, Bartolucci AA, Hunter GR, Figueroa-Colon R, Goran MI, A longitudinal study of resting energy expenditure relative to body composition during puberty in African American and white children. Am J Clin Nutr73(2):308-15 2001 |
| PubMed ID: 11157329 |
| |
| Goran MI, Visceral fat in prepubertal children: Influence of obesity, anthropometry, ethnicity, gender, diet, and growth. Am J Human Biol11(2):201-207 1999 |
| PubMed ID: 11533944 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
|
|