AG17907
Adipose stromal cell from Skin, Thigh
Description:
APPARENTLY HEALTHY INDIVIDUAL
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Apparently Healthy Collection |
|
Biopsy Source
|
Thigh
|
|
Cell Type
|
Adipose stromal cell
|
|
Tissue Type
|
Fat
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Adipose stromal cell from Skin, Thigh
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
46,XX[22]
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Senescence |
8 |
| Passage Frozen |
2 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
The fat tissue was removed from the subject's inner thighs and knees ante-mortem during elective cosmetic tumescent liposuction on 9/13/02. This primary culture was initiated on 9/13/02 by collagenase digestion of fat tissue. Donor subject weighed 138 lbs (62.6 kgs) at the time of the procedure. This culture tested negative for the endothelial cell-specific von Willebrand factor, negative for alpha-smooth muscle actin and positive for Cy3/Vimentin (immunofluorescence assays). 50% of the cells observed at passage 4 showed adipogenic differentiation. THIS SAMPLE WILL ONLY BE SHIPPED FROZEN. |
| PDL at Senescence |
8 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Adipose Stromal Cell Medium |
| Serum |
0% fetal bovine serum Not inactivated |
| Substrate |
Human Fibronectin |
| Supplement |
- |
|
|