AG19304
Adipose stromal cell from Skin, Abdomen
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Abdomen
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Cell Type
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Adipose stromal cell
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Tissue Type
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Fat
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Transformant
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Untransformed
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Sample Source
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Adipose stromal cell from Skin, Abdomen
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
22 |
| Passage Frozen |
0 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
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| Remarks |
The fat tissue was removed from the subject's abdomen and flanks ante-mortem during elective cosmetic tumescent liposuction on 1/30/04. This primary culture was initiated on 1/30/04 by collagenase digestion of fat tissue. Donor subject (5 ft 8 in) weighed 160 lbs (72.5 kgs) at the time of the procedure. This culture tested negative for the endothelial cell-specific von Willebrand factor, negative for alpha-smooth muscle actin and positive for Cy3/Vimentin (immunofluorescence assays). 56% (at passage 3) and 7% (at passage 9) of the cells observed showed adipogenic differentiation. THIS SAMPLE WILL ONLY BE SHIPPED FROZEN. |
| PDL at Senescence |
22 |
| Passage Frozen |
0 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Adipose Stromal Cell Medium |
| Serum |
0% fetal bovine serum Not inactivated |
| Substrate |
Human Fibronectin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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