AG20367
Adipose stromal cell from Skin, Thigh
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Thigh
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Cell Type
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Adipose stromal cell
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Tissue Type
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Fat
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Transformant
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Untransformed
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Sample Source
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Adipose stromal cell from Skin, Thigh
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
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| Remarks |
The fat tissue was removed from the subject's outer thighs ("saddle bags") ante-mortem during elective cosmetic tumescent liposuction on 2/20/02. This primary culture was initiated on 2/20/02 by collagenase digestion of fat tissue. This culture tested negative for the endothelial cell-specific von Willebrand factor, negative for alpha-smooth muscle actin and positive for Cy3/Vimentin (immunofluorescence assays). 41.6% of the cells observed at passage 5 showed adipogenic differentiation. THIS SAMPLE WILL ONLY BE SHIPPED FROZEN. |
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