AG20368
Adipose stromal cell from Skin, Abdomen
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Abdomen
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Cell Type
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Adipose stromal cell
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Tissue Type
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Fat
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Transformant
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Untransformed
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Sample Source
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Adipose stromal cell from Skin, Abdomen
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
4.12 |
| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
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| Remarks |
The fat tissue was removed from the subject's abdomen ante-mortem during elective cosmetic tumescent liposuction on 3/22/02. This primary culture was initiated on 3/22/02 by collagenase digestion of fat tissue. This culture tested negative for the endothelial cell-specific von Willebrand factor, negative for alpha-smooth muscle actin and positive for Cy3/Vimentin (immunofluorescence assays). 13.8% of the cells observed at passage 5 showed adipogenic differentiation. THIS SAMPLE WILL ONLY BE SHIPPED FROZEN. |
| Cumulative PDL at Freeze |
4.12 |
| Passage Frozen |
3 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Human Fibronectin |
| Supplement |
- |
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