AG21837
Keratinocyte from Skin, Foreskin
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Foreskin
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Cell Type
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Keratinocyte
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Keratinocyte from Skin, Foreskin
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Family Member
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1
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
35 |
| PDL at Freeze |
2.33 |
| Passage Frozen |
1 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
Foreskin keratinocyte cell culture; matching foreskin melanocyte and foreskin fibroblast cultures from this same donor are AG21838 and AG21839, respectively. Must ship frozen. |
| Velarde MC, Flynn JM, Day NU, Melov S, Campisi J., Mitochondrial oxidative stress caused by Sod2 deficiency promotes cellular senescence and aging phenotypes in the skin. Aging4:3-12 2012 |
| PubMed ID: 22278880 |
| PDL at Senescence |
35 |
| Cumulative PDL at Freeze |
2.33 |
| Passage Frozen |
1 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
CnT-07 Epidermal Progenitor Cell Medium |
| Serum |
0% none |
| Substrate |
Collagen IV-coated |
| Supplement |
- |
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