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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Relation to Proband
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proband
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|
Confirmation
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Clinical summary/Case history
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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|
| Passage Frozen |
8 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Genbank |
COX1 |
| |
| Genbank |
COX1 |
| Remarks |
Line B-82, mouse L cell; fibroblastlike; thymidine kinase deficient; selectable with 5-bromodeoxyuridine; heteroploid |
| Maraia RJ, Sasaki-Tozawa N, Driscoll CT, Green ED, Darlington GJ, The human Y4 small cytoplasmic RNA gene is controlled by upstream elements and resides on chromosome 7 with all other hY scRNA genes Nucleic acids research22:3045-52 1994 |
| PubMed ID: 7520568 |
| |
| Dorig RE, Marcil A, Chopra A, Richardson CD, The human CD46 molecule is a receptor for measles virus (Edmonston strain). Cell75:295-305 1993 |
| PubMed ID: 8402913 |
| |
| Salido EC, Passage MB, Yen PH, Shapiro LJ, Mohandas TK, An evaluation of the inactive mouse X chromosome in somatic cell hybrids. Somat Cell Mol Genet19:65-71 1993 |
| PubMed ID: 8460399 |
| |
| Sparkes RS, Heinzmann C, Goldflam S, Kojis T, Saari JC, Mohandas T, Klisak I, Bateman JB, Crabb JW, Assignment of the gene (RLBP1) for cellular retinaldehyde-binding protein (CRALBP) to human chromosome 15q26 and mouse chromosome 7. Genomics12:58-62 1992 |
| PubMed ID: 1733864 |
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| Lau EC, Mohandas TK, Shapiro LJ, Slavkin HC, Snead ML, Human and mouse amelogenin gene loci are on the sex chromosomes. Genomics4:162-8 1989 |
| PubMed ID: 2737677 |
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| Bols NC, Mosser DD, Boliska SA, Transfer of purine metabolites between cells through the medium and via cell contacts in cocultures of HGPRT+ and HGPRT- cells. Exp Cell Res173:206-17 1987 |
| PubMed ID: 3678380 |
| |
| Taggart RT, Mohandas TK, Bell GI, Parasexual analysis of human pepsinogen molecular heterogeneity. Somat Cell Mol Genet13:167-72 1987 |
| PubMed ID: 3031827 |
| |
| Hsu LC, Yoshida A, Mohandas T, Chromosomal assignment of the genes for human aldehyde dehydrogenase-1 and aldehyde dehydrogenase-2. Am J Hum Genet38:641-8 1986 |
| PubMed ID: 3013004 |
| |
| Lusis AJ, Heinzmann C, Sparkes RS, Scott J, Knott TJ, Geller R, Sparkes MC, Mohandas T, Regional mapping of human chromosome 19: organization of genes for plasma lipid transport (APOC1, -C2, and -E and LDLR) and the genes C3, PEPD, and GPI. Proc Natl Acad Sci U S A83:3929-33 1986 |
| PubMed ID: 3459164 |
| |
| Mohandas T, Heinzmann C, Sparkes RS, Wasmuth J, Edwards P, Lusis AJ, Assignment of human 3-hydroxy-3-methylglutaryl coenzyme A reductase gene to q13----q23 region of chromosome 5. Somat Cell Mol Genet12:89-94 1986 |
| PubMed ID: 3456176 |
| |
| Sparkes RS, Dizikes GJ, Klisak I, Grody WW, Mohandas T, Heinzmann C, Zollman S, Lusis AJ, Cederbaum SD, The gene for human liver arginase (ARG1) is assigned to chromosome band 6q23. Am J Hum Genet39:186-93 1986 |
| PubMed ID: 3752085 |
| |
| Sparkes RS, Simon M, Cohn VH, Fournier RE, Lem J, Klisak I, Heinzmann C, Blatt C, Lucero M, Mohandas T, et al, Assignment of the human and mouse prion protein genes to homologous chromosomes. Proc Natl Acad Sci U S A83:7358-62 1986 |
| PubMed ID: 3094007 |
| |
| Taggart RT, Mohandas TK, Shows TB, Bell GI, Variable numbers of pepsinogen genes are located in the centromeric region of human chromosome 11 and determine the high-frequency electrophoretic polymorphism. Proc Natl Acad Sci U S A82:6240-4 1985 |
| PubMed ID: 3862130 |
| |
| Mohandas T, Sparkes RS, Suh EJ, Hershfield MS, Regional localization of the human genes for S-adenosylhomocysteine hydrolase (cen----q131) and adenosine deaminase (q131----qter) on chromosome 20. Hum Genet66:292-5 1984 |
| PubMed ID: 6586634 |
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| O'Donnell RW, Horan PK, Minken TJ, Chuang C, Henshaw EC, McCune CS, Antitumor immunity induced by hybrid tumor cells: comparison between hybrids and the parental tumor. Cancer Res44:487-92 1984 |
| PubMed ID: 6318971 |
| |
| Boliska SA, Bols NC, The transfer of purine metabolites via the medium rather than through cell contacts. Exp Cell Res141:303-12 1982 |
| PubMed ID: 7140841 |
| |
| Hwang SP, Kucherlapati R, Localization and organization of integrated simian virus 40 sequences in a human cell line. Virology105:196-204 1980 |
| PubMed ID: 6251605 |
| |
| Dorman BP, Shimizu N, Ruddle FH, Genetic analysis of the human cell surface: antigenic marker for the human X chromosome in human-mouse hybrids. Proc Natl Acad Sci U S A75:2363-7 1978 |
| PubMed ID: 353811 |
| |
| Sundar Raj CV, Church RL, Klobutcher LA, Ruddle FH, Genetics of the connective tissue proteins: assignment of the gene for human type I procollagen to chromosome 17 by analysis of cell hybrids and microcell hybrids. Proc Natl Acad Sci U S A74:4444-8 1977 |
| PubMed ID: 412188 |
| |
| Littlefield JW, The use of drug-resistant markers to study the hybridization of mouse fibroblasts. Exp Cell Res41:190-6 1966 |
| PubMed ID: 4952025 |
| Passage Frozen |
8 |
| Split Ratio |
1:25 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|