GM03905
Endothelial from Aorta, thoracic
Description:
BOVINE ENDOTHELIAL CELL CULTURES
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| PDL at Senescence |
79 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Clinically normal; male; 6 months gestation; aortic endothelial cell culture; received at PDL 53 and passaged 6 more times at CCR; estimated replicative life-span is 80 PDL; clone BFA-1c |
| Peng X, Haldar S, Deshpande S, Irani K, Kass DA, Wall stiffness suppresses Akt/eNOS and cytoprotection in pulse-perfused
endothelium. Hypertension41(2):378-81 2003 |
| PubMed ID: 12574111 |
| |
| Panetti TS, Nowlen J, Mosher DF, Sphingosine-1-phosphate and lysophosphatidic acid stimulate endothelial cell migration. Arterioscler Thromb Vasc Biol20(4):1013-9 2000 |
| PubMed ID: 10764666 |
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| Friedberg RC, Hagen PO, Pizzo SV, The role of endothelium in factor Xa regulation: the effect of plasma proteinase inhibitors and hirudin. Blood71:1321-8 1988 |
| PubMed ID: 3162814 |
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| Murray JJ, Fridovich I, Makhoul RG, Hagen PO, Stabilization and partial characterization of endothelium-derived relaxing factor from cultured bovine aortic endothelial cells. Biochem Biophys Res Commun141:689-96 1986 |
| PubMed ID: 3492205 |
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| Grinspan JB, Mueller SN, Levine EM, Bovine endothelial cells transformed in vitro by benzo(a)pyrene. J Cell Physiol114:328-38 1983 |
| PubMed ID: 6833405 |
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| Mueller SN, Rosen EM, Levine EM, Cellular senescence in a cloned strain of bovine fetal aortic endothelial cells. Science207:889-91 1980 |
| PubMed ID: 7355268 |
| PDL at Senescence |
79 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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