GM07299
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/CHINESE HAMSTER SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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1
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Chinese hamster/human hybrid retaining human chromosomes #1 & X
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for Xp22.3p22.2, DXS85, Xq28, DXS15, and Xp22.32 and Yp11.3, MIC2. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp22.32, STS, Xq13.3, PGK1, and Xq28, G6PD. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp22.32, STS, Xq13.3, PGK1, Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 1p36, PND; 1p36 (also 3p21), DNF15S1/S2; and 1q32, REN. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 1p13, NGFB, and 1q32, REN. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, 2p23, POMC, and 2q13, Il1A. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 2q37, ALPP. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 3p25, RAF1. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3p22-p21.33, GLB1, and 3q26.2-q27, GLUT2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 4p15.3, QDPR, and 4q11-q13, AFP. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, and 5q13.3-q14, HMGCR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 6q24q27, ESR. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, and 6q24-q27, MAS1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 7pterp15, ASSP11. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 7p12, EGFR, and 7q31.3, CFTR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 8p21-p11.2, LHRH, 8p23-p22, DEF1, and 8q24, TG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 9p22, IFNB. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p22, IFNA, and 9q21.3-q22.2, ALDOB. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p21, D9S270, 9pter-p22, D9S178, and 9pter-q12, RLN1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 10p11.2, ITGB1, and 10q24, PLAU. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 11q23.3, ETS1. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 11p13, CAT, and 11q21-q22, CLG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 12p13.2, PRB1, and 12q14q24.1, D12S7. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 12p13.3-p13.2, F8VWF, and 12q22-q24.2, PAH. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 13q34, D13S3. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 13q14.3, RB1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 14q11.2, NP, and 14q32.1, PI. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 15q25qter, IGF1R. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q21-q22.2, B2M. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q11.2-q12, GABRB3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 16q13q22.1, MT2. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 16p13.3, HBA, 16q22.1, HPR, and 16q22.1, LCAT. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 17p13.1, MYH2, and 17q21.3q23, MPO. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 17p13.1, TP53, 17q, GAS, and 17q21.3-q23, MPO. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 18p11.31-p11.22, TYMS. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 18p11.1-q11.2, D18S44, and 18q12.1, TTR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 19p13.2, LDLR, and 19q13.2, APOC2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 20 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 20pter-p12, PRNP, and 20q12-q13.11, ADA. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 21q22.1q22.2, D21S17. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 21q22.3, ITGB2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 22q11.2, IGLC2. |
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| Remarks |
Human/HPRT-deficient Chinese hamster lung cell line; hybrid #MR 8.2; selected in medium containing azaserine; contains human chromosomes #1 and X; at P7, X is present in 100% of cells and chromosome #1 is present in 72% of cells |
| Katsanis N, Fisher EM, A novel C-terminal binding protein (CTBP2) is closely related to CTBP1, an adenovirus E1A-binding protein, and maps to human chromosome 21q21.3. Genomics47:294-9 1998 |
| PubMed ID: 9479502 |
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| Campbell HD, Webb GC, Fountain S, Young IG, The human PIN1 peptidyl-prolyl cis/trans isomerase gene maps to human chromosome 19p13 and the closely related PIN1L gene to 1p31. Genomics44:157-62 1997 |
| PubMed ID: 9299231 |
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| Cowley CM, Simrak D, Spurr NK, Arnemann J, Buxton RS, The plakophilin 1 (PKP1) and plakoglobin (JUP) genes map to human chromosomes 1q and 17, respectively [letter] Hum Genet100:486-8 1997 |
| PubMed ID: 9272178 |
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| Jones MH, Zhang Y, Tirosvoutis KN, Davey PM, Webster AR, Walsh D, Spurr NK, Affara NA, Chromosomal assignment of 311 sequences transcribed in human adult testis. Genomics40:155-67 1997 |
| PubMed ID: 9070934 |
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| Liang Y, Chen H, Asher JH Jr, Chang CC, Friedman TB, Human inner ear OCP2 cDNA maps to 5q22-5q35.2 with related sequences on chromosomes 4p16.2-4p14, 5p13-5q22, 7pter-q22, 10 and 12p13-12qter. Gene184:163-7 1997 |
| PubMed ID: 9031623 |
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| McCusker D, Jones T, Sheer D, Trowsdale J, Genetic relationships of the genes encoding the human proteasome beta subunits and the proteasome PA28 complex. Genomics45:362-7 1997 |
| PubMed ID: 9344661 |
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| Hardas BD, Zhao X, Zhang J, Longqing X, Stoll S, Elder JT, Assignment of psoriasin to human chromosomal band 1q21: coordinate overexpression of clustered genes in psoriasis. J Invest Dermatol106:753-8 1996 |
| PubMed ID: 8618016 |
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| McCombie RR, Dolphin CT, Povey S, Phillips IR, Shephard EA, Localization of human flavin-containing monooxygenase genes FMO2 and FMO5 to chromosome 1q. Genomics34:426-9 1996 |
| PubMed ID: 8786146 |
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| Aguado B, Campbell RD, The novel gene G17, located in the human major histocompatibility complex, encodes PBX2, a homeodomain-containing protein. Genomics25:650-9 1995 |
| PubMed ID: 7759099 |
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| Kelsell DP, Rooke L, Warne D, Bouzyk M, Cullin L, Cox S, West L, Povey S, Spurr NK, Development of a panel of monochromosomal somatic cell hybrids for rapid gene mapping Annals of human genetics59:233-41 1995 |
| PubMed ID: 7625768 |
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| Saito M, Helin K, Valentine MB, Griffith BB, Willman CL, Harlow E, Look AT, Amplification of the E2F1 transcription factor gene in the HEL erythroleukemia cell line. Genomics25:130-8 1995 |
| PubMed ID: 7774910 |
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| Yulug IG, Egan SE, See CG, Fisher EM, A human SHC-related sequence maps to chromosome 17, the SHC gene maps to chromosome 1. Hum Genet96:245-8 1995 |
| PubMed ID: 7635484 |
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| Chang DY, Nelson B, Bilyeu T, Hsu K, Darlington GJ, Maraia RJ, A human Alu RNA-binding protein whose expression is associated with accumulation of small cytoplasmic Alu RNA. Mol Cell Biol14:3949-59 1994 |
| PubMed ID: 8196634 |
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| Driscoll CT, Darlington GJ, Maraia RJ, The conserved 7SK snRNA gene localizes to human chromosome 6 by homolog
exclusion probing of somatic cell hybrid RNA. Nucleic Acids Res22(5):722-5 1994 |
| PubMed ID: 8139910 |
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| Hendershot LM, Valentine VA, Lee AS, Morris SW, Shapiro DN, Localization of the gene encoding human BiP/GRP78, the endoplasmic reticulum cognate of the HSP70 family, to chromosome 9q34. Genomics20:281-4 1994 |
| PubMed ID: 8020977 |
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| Huebner K, Kastury K, Druck T, Salcini AE, Lanfrancone L, Pelicci G, Lowenstein E, Li W, Park SH, Cannizzaro L, et al, Chromosome locations of genes encoding human signal transduction adapter proteins, Nck (NCK), Shc (SHC1), and Grb2 (GRB2). Genomics22:281-7 1994 |
| PubMed ID: 7806213 |
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| Kass S, MacRae C, Graber HL, Sparks EA, McNamara D, Boudoulas H, Basson CT, Baker PB 3rd, Cody RJ, Fishman MC, et al, A gene defect that causes conduction system disease and dilated cardiomyopathy maps to chromosome 1p1-1q1. Nat Genet7:546-51 1994 |
| PubMed ID: 7951328 |
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| Kastury K, Druck T, Huebner K, Barletta C, Acampora D, Simeone A, Faiella A, Boncinelli E, Chromosome locations of human EMX and OTX genes. Genomics22:41-5 1994 |
| PubMed ID: 7959790 |
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| Maraia RJ, Sasaki-Tozawa N, Driscoll CT, Green ED, Darlington GJ, The human Y4 small cytoplasmic RNA gene is controlled by upstream elements and resides on chromosome 7 with all other hY scRNA genes Nucleic acids research22:3045-52 1994 |
| PubMed ID: 7520568 |
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| Seltmann M, Bohm M, Walter T, Schmitz G, Patterson D, Wieland I, Isolation of region specific single-copy probes from human chromosome 1q23-->1q25. Cytogenet Cell Genet67:46-51 1994 |
| PubMed ID: 8187551 |
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| Clark CC, Cohen I, Eichstetter I, Cannizzaro LA, McPherson JD, Wasmuth JJ, Iozzo RV, Molecular cloning of the human proto-oncogene Wnt-5A and mapping of the gene (WNT5A) to chromosome 3p14-p21. Genomics18:249-60 1993 |
| PubMed ID: 8288227 |
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| Dorig RE, Marcil A, Chopra A, Richardson CD, The human CD46 molecule is a receptor for measles virus (Edmonston strain). Cell75:295-305 1993 |
| PubMed ID: 8402913 |
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| Drwinga HL, Toji LH, Kim CH, Greene AE, Mulivor RA, NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2. Genomics16:311-4 1993 |
| PubMed ID: 8314568 |
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| Figlewicz DA, Delattre O, Guellaen G, Krizus A, Thomas G, Zucman J, Rouleau GA, Mapping of human gamma-glutamyl transpeptidase genes on chromosome 22 and other human autosomes. Genomics17:299-305 1993 |
| PubMed ID: 8104871 |
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| Maraia RJ, Driscoll CT, Bilyeu T, Hsu K, Darlington GJ, Multiple dispersed loci produce small cytoplasmic Alu RNA. Mol Cell Biol13:4233-41 1993 |
| PubMed ID: 7686619 |
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| Morris SW, Valentine MB, Kirstein MN, Huebner K, Reassignment of the human ARH9 RAS-related gene to chromosome 1p13-p21. Genomics15:677-9 1993 |
| PubMed ID: 8468062 |
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| Washington SS, Bowcock AM, Gerken S, Matsunami N, Lesh D, Osborne-Lawrence SL, Cowell J, Ledbetter DH, White RL, Chakravarti A, A somatic cell hybrid map of human chromosome 13. Genomics18:486-95 1993 |
| PubMed ID: 8307557 |
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| Morris SW, Nelson N, Valentine MB, Shapiro DN, Look AT, Kozlosky CJ, Beckmann MP, Cerretti DP, Assignment of the genes encoding human interleukin-8 receptor types 1 and 2 and an interleukin-8 receptor pseudogene to chromosome 2q35. Genomics14:685-91 1992 |
| PubMed ID: 1427896 |
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| Finger LR, Kagan J, Christopher G, Kurtzberg J, Hershfield MS, Nowell PC, Croce CM, Involvement of the TCL5 gene on human chromosome 1 in T-cell leukemia and melanoma. Proc Natl Acad Sci U S A86:5039-43 1989 |
| PubMed ID: 2740341 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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