GM07372
Endothelial from Aorta, thoracic
Description:
BOVINE ENDOTHELIAL CELL CULTURES
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Animals |
|
Biopsy Source
|
Aorta, thoracic
|
|
Cell Type
|
Endothelial
|
|
Tissue Type
|
Artery
|
|
Transformant
|
Benzo(a)pyrene
|
|
Sample Source
|
Endothelial from Aorta, thoracic
|
|
Family Member
|
2
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Bos taurus
|
|
Common Name
|
cow
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Clinically normal male; 6 months gestation; aortic endothelial cell culture transformed in vitro with benzo(a)pyrene; BFA-1c 1BPT flat transformants; submitted at PDL 283; non-tumorigenic in nude mice |
| Del Vecchio RL, Tonks NK, The conserved immunoglobulin domain controls the subcellular localization of the homophilic adhesion receptor protein-tyrosine phosphatase mu The Journal of biological chemistry280:1603-12 2004 |
| PubMed ID: 15491993 |
| |
| Peng X, Haldar S, Deshpande S, Irani K, Kass DA, Wall stiffness suppresses Akt/eNOS and cytoprotection in pulse-perfused
endothelium. Hypertension41(2):378-81 2003 |
| PubMed ID: 12574111 |
| |
| Panetti TS, Nowlen J, Mosher DF, Sphingosine-1-phosphate and lysophosphatidic acid stimulate endothelial cell migration. Arterioscler Thromb Vasc Biol20(4):1013-9 2000 |
| PubMed ID: 10764666 |
| |
| Escargueil-Blanc I, Meilhac O, Pieraggi MT, Arnal JF, Salvayre R, Negre-Salvayre A, Oxidized LDLs induce massive apoptosis of cultured human endothelial cells through a calcium-dependent pathway. Prevention by aurintricarboxylic acid. Arterioscler Thromb Vasc Biol17:331-9 1997 |
| PubMed ID: 9081689 |
| |
| Suc I, Escargueil-Blanc I, Troly M, Salvayre R, Negre-Salvayre A, HDL and ApoA prevent cell death of endothelial cells induced by oxidized LDL. Arterioscler Thromb Vasc Biol17:2158-66 1997 |
| PubMed ID: 9351385 |
| |
| Bourguignon LY, Iida N, Sobrin L, Bourguignon GJ, Identification of an IP3 receptor in endothelial cells. J Cell Physiol159:29-34 1994 |
| PubMed ID: 8138588 |
| |
| Grinspan JB, Mueller SN, Levine EM, Bovine endothelial cells transformed in vitro by benzo(a)pyrene. J Cell Physiol114:328-38 1983 |
| PubMed ID: 6833405 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|