GM10324
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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23
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/Mouse somatic cell hybrid retaining the human X chromosome in 90% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for Xp22.2, DXS16, and Xq28, DXS15. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp11.3, DXS1003, Xp22.32, STS, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 3q26.2qter, TFRC. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 5pterp15.3, D5S10. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 12p13, TPI1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 16pterp13, D16S36, and 16q13q22.1, MT2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 18q23, MBP. |
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| Remarks |
Line AHA 11a; produced by fusing human fibroblasts, GM00144, 46,XX,-6,+der(6) t(6;21), with the HPRT- and APRT-deficient mouse cell line A9; selected in HAT medium; 90% of cells retain the human X chromosome |
| Coskun AK, van Maanen M, Nguyen V, Sutton RE, Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1 Journal of virology80:3406-15 2006 |
| PubMed ID: 16537608 |
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| Kubota T, Nonoyama S, Tonoki H, Masuno M, Imaizumi K, Kojima M, Wakui K,
Shimadzu M, Fukushima Y, A new assay for the analysis of X-chromosome inactivation based on
methylation-specific PCR. Hum Genet104(1):49-55 1999 |
| PubMed ID: 10071192 |
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| Swanson DA, Freund CL, Ploder L, McInnes RR, Valle D, A ubiquitin C-terminal hydrolase gene on the proximal short arm of the X chromosome: implications for X-linked retinal disorders Human molecular genetics5:533-8 1996 |
| PubMed ID: 8845848 |
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| Bloch KD, Wolfram JR, Brown DM, Roberts JD Jr, Zapol DG, Lepore JJ, Filippov G, Thomas JE, Jacob HJ, Bloch DB, Three members of the nitric oxide synthase II gene family (NOS2A, NOS2B, and NOS2C) colocalize to human chromosome 17. Genomics27:526-30 1995 |
| PubMed ID: 7558036 |
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| Saito M, Helin K, Valentine MB, Griffith BB, Willman CL, Harlow E, Look AT, Amplification of the E2F1 transcription factor gene in the HEL erythroleukemia cell line. Genomics25:130-8 1995 |
| PubMed ID: 7774910 |
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| Fukushima A, Okubo K, Sugino H, Hori N, Matoba R, Niiyama T, Murakawa K, Yoshii J, Yokoyama M, Matsubara K, Chromosomal assignment of HepG2 3'-directed partial cDNA sequences by Southern blot hybridization using monochromosomal hybrid cell panels. Genomics22:127-36 1994 |
| PubMed ID: 7959758 |
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| Hendershot LM, Valentine VA, Lee AS, Morris SW, Shapiro DN, Localization of the gene encoding human BiP/GRP78, the endoplasmic reticulum cognate of the HSP70 family, to chromosome 9q34. Genomics20:281-4 1994 |
| PubMed ID: 8020977 |
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| Drwinga HL, Toji LH, Kim CH, Greene AE, Mulivor RA, NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2. Genomics16:311-4 1993 |
| PubMed ID: 8314568 |
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| Howe JR, Lairmore TC, Veile R, Dou S, Wells SA Jr, Donis-Keller H, Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping. Hum Genet91:199-204 1993 |
| PubMed ID: 8478002 |
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| Morris SW, Nelson N, Valentine MB, Shapiro DN, Look AT, Kozlosky CJ, Beckmann MP, Cerretti DP, Assignment of the genes encoding human interleukin-8 receptor types 1 and 2 and an interleukin-8 receptor pseudogene to chromosome 2q35. Genomics14:685-91 1992 |
| PubMed ID: 1427896 |
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| Dorman BP, Shimizu N, Ruddle FH, Genetic analysis of the human cell surface: antigenic marker for the human X chromosome in human-mouse hybrids. Proc Natl Acad Sci U S A75:2363-7 1978 |
| PubMed ID: 353811 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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