GM10479
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/mouse somatic cell hybrid retaining human chromosome #14
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for Xp22.2, DXS16; Xp22.32 (also Yp11.3), MIC2; and Xq28, DXS15. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xp22.32, STS, Xq13.3, PGK1, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 1p13, NGFB, and 1q32, REN. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 2p25, D2S1. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, 2p23, POMC, and 2q13, IL1A. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3p22-p21.33, GLB1, and 3q26.2-q27, GLUT2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 4p15.3, QDPR, and 4q11-q13, AFP. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 5q33q35, PDGFRB. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, and 5q13.3-q14, HMGCR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, and 6q24-q27, MAS1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 7p12, EGFR, and 7q31.3, CFTR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 8p21-p11.2, LHRH, 8p23-p22, DEF1, and 8q24, TG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p22, IFNA, and 9q22.3-q31, ALDOB. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 10q24qter, PLAU. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 10p11.2, ITGB1, and 10q24, PLAU. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 11p13, CAT, and 11q21-q22, CLG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 12q14q24.1, D12S7. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 12p13.3-p13.2, F8VWF, and 12q22-q24.2, PAH. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 13q14.3, RB1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 14q32.33, IGHG4, and 14q32.1q32.3, D14S13. Results from two different laboratories (personal communications) employing either pulsed-field electrophoresis or Southern blot hybridization have demonstrated an absence of human rDNA for this hybrid cell culture. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 14q11.2, NP, and 14q32.1, PI. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 14q11.2, NP, and 14q32.1, PI. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q21-q22.2, B2M. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 16pterp13, D16S36, and 16q13q22.1, MT2. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 16p13.3, HBA, 16q22.1, HPR, and 16q22.1, LCAT. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 16p13.3, HBA, and gave positive results with primers for 16q22.1, LCAT, 16q23.2, D16S266, and 16q24.2, D16S389. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 17q21.3q23, MPO, and 17p13.1, MYH2. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 17p13.1, TP53, 17q, GAS, and 17q21.3-q23, MPO. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 18p11.31-p11.22, TYMS. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 19p13.2, LDLR, and 19q13.2, APOC2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 20 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 20pter-p12, PRNP, and 20q12-q13.11, ADA. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 21q11, D21S26, and 21q22.1q22.2, D21S17. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 21q22.3, ITGB2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 22q11.2, IGLC2. |
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| Remarks |
Line HDm-5; produced by microcell-mediated chromosome transfer of human foreskin fibroblast chromosomes with retroviral vector insertions and mouse 3T6 cells; selected in medium with geneticin (0.25 mg/ml); 96% of cells retain human chromosome #14 |
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| PubMed ID: 24838473 |
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| Coskun AK, van Maanen M, Nguyen V, Sutton RE, Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1 Journal of virology80:3406-15 2006 |
| PubMed ID: 16537608 |
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| Quade BJ, Weremowicz S, Neskey DM, Vanni R, Ladd C, Dal Cin P, Morton CC, Fusion transcripts involving HMGA2 are not a common molecular mechanism in
uterine leiomyomata with rearrangements in 12q15. Cancer Res63(6):1351-8 2003 |
| PubMed ID: 12649198 |
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| Anderson MJ, Viars CS, Czekay S, Cavenee WK, Arden KC, Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics47:187-99 1998 |
| PubMed ID: 9479491 |
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| Dear PH, Bankier AT, Piper MB, A high-resolution metric HAPPY map of human chromosome 14. Genomics48:232-41 1998 |
| PubMed ID: 9521877 |
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| Hisama FM, Oshima J, Yu CE, Fu YH, Mulligan J, Weissman SM, Schellenberg GD, Comparison of methods for identifying transcription units and transcription map of the Werner syndrome gene region. Genomics52:342-51 1998 |
| PubMed ID: 9790753 |
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| Katsanis N, Fisher EM, A novel C-terminal binding protein (CTBP2) is closely related to CTBP1, an adenovirus E1A-binding protein, and maps to human chromosome 21q21.3. Genomics47:294-9 1998 |
| PubMed ID: 9479502 |
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| Biunno I, Appierto V, Cattaneo M, Leone BE, Balzano G, Socci C, Saccone S, Letizia A, Della Valle G, Sgaramella V, Isolation of a pancreas-specific gene located on human chromosome 14q31: expression analysis in human pancreatic ductal carcinomas. Genomics46:284-6 1997 |
| PubMed ID: 9417916 |
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| Dyomin VG, Rao PH, Dalla-Favera R, Chaganti RS, BCL8, a novel gene involved in translocations affecting band 15q11-13 in diffuse large-cell lymphoma. Proc Natl Acad Sci U S A94(11):5728-32 1997 |
| PubMed ID: 9159141 |
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| McCusker D, Jones T, Sheer D, Trowsdale J, Genetic relationships of the genes encoding the human proteasome beta subunits and the proteasome PA28 complex. Genomics45:362-7 1997 |
| PubMed ID: 9344661 |
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| Schwarz H, Arden K, Lotz M, CD137, a member of the tumor necrosis factor receptor family, is located on chromosome 1p36, in a cluster of related genes, and colocalizes with several malignancies Biochemical and biophysical research communications235:699-703 1997 |
| PubMed ID: 9207223 |
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| Hirai M, Kusuda J, Hashimoto K, Assignment of human ADP ribosylation factor (ARF) genes ARF1 and ARF3 to chromosomes 1q42 and 12q13, respectively. Genomics34(2):263-5 1996 |
| PubMed ID: 8661066 |
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| Walter CA, Shaffer LG, Kaye CI, Huff RW, Ghidoni PD, McCaskill C, McFarland MB, Moore CM, Short-limb dwarfism and hypertrophic cardiomyopathy in a patient with paternal isodisomy 14: 45,XY,idic(14)(p11). Am J Med Genet65:259-65 1996 |
| PubMed ID: 8923931 |
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| Arden KC, Viars CS, Weiss S, Argentin S, Nemer M, Localization of the human B-type natriuretic peptide precursor (NPPB) gene to chromosome 1p36. Genomics26:385-9 1995 |
| PubMed ID: 7601467 |
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| Bermingham JR Jr, Arden KC, Naumova AK, Sapienza C, Viars CS, Fu XD, Khotz J, Manley JL, Rosenfeld MG, Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2). Genomics29:70-9 1995 |
| PubMed ID: 8530103 |
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| Kelsell DP, Rooke L, Warne D, Bouzyk M, Cullin L, Cox S, West L, Povey S, Spurr NK, Development of a panel of monochromosomal somatic cell hybrids for rapid gene mapping Annals of human genetics59:233-41 1995 |
| PubMed ID: 7625768 |
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| Kermouni A, Van Roost E, Arden KC, Vermeesch JR, Weiss S, Godelaine D, Flint J, Lurquin C, Szikora JP, Higgs DR, The IL-9 receptor gene (IL9R): genomic structure, chromosomal localization in the pseudoautosomal region of the long arm of the sex chromosomes, and identification of IL9R pseudogenes at 9qter, 10pter, 16pter, and 18pter Genomics29:371-82 1995 |
| PubMed ID: 8666384 |
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| Lyle R, Wright TJ, Clark LN, Hewitt JE, The FSHD-associated repeat, D4Z4, is a member of a dispersed family of homeobox-containing repeats, subsets of which are clustered on the short arms of the acrocentric chromosomes. Genomics28:389-97 1995 |
| PubMed ID: 7490072 |
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| Saito M, Helin K, Valentine MB, Griffith BB, Willman CL, Harlow E, Look AT, Amplification of the E2F1 transcription factor gene in the HEL erythroleukemia cell line. Genomics25:130-8 1995 |
| PubMed ID: 7774910 |
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| Sakai K, Ohta T, Minoshima S, Kudoh J, Wang Y, de Jong PJ, Shimizu N, Human ribosomal RNA gene cluster: identification of the proximal end containing a novel tandem repeat sequence. Genomics26:521-6 1995 |
| PubMed ID: 7607675 |
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| Sherrington R, Rogaev EI, Liang Y, Rogaeva EA, Levesque G, Ikeda M, Chi H, Lin C, Li G, Holman K, et al, Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease [see comments] Nature375:754-60 1995 |
| PubMed ID: 7596406 |
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| Carey ML, Liang Y, Barber TD, Morell R, Johnson DH, Cox S, Asher JH Jr, Friedman TB, Dinucleotide repeat polymorphism at D14S542. Hum Mol Genet3:1712 1994 |
| PubMed ID: 7833943 |
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| Chang DY, Nelson B, Bilyeu T, Hsu K, Darlington GJ, Maraia RJ, A human Alu RNA-binding protein whose expression is associated with accumulation of small cytoplasmic Alu RNA. Mol Cell Biol14:3949-59 1994 |
| PubMed ID: 8196634 |
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| Cox DW, Report of the first international workshop on human chromosome 14 mapping 1993 Cytogenetics and cell genetics66:2-9 1994 |
| PubMed ID: 8275701 |
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| De Plaen E, Arden K, Traversari C, Gaforio JJ, Szikora JP, De Smet C, Brasseur F, van der Bruggen P, Lethé B, Lurquin C, Structure, chromosomal localization, and expression of 12 genes of the MAGE family Immunogenetics40:360-9 1994 |
| PubMed ID: 7927540 |
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| Driscoll CT, Darlington GJ, Maraia RJ, The conserved 7SK snRNA gene localizes to human chromosome 6 by homolog
exclusion probing of somatic cell hybrid RNA. Nucleic Acids Res22(5):722-5 1994 |
| PubMed ID: 8139910 |
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| Fukushima A, Okubo K, Sugino H, Hori N, Matoba R, Niiyama T, Murakawa K, Yoshii J, Yokoyama M, Matsubara K, Chromosomal assignment of HepG2 3'-directed partial cDNA sequences by Southern blot hybridization using monochromosomal hybrid cell panels. Genomics22:127-36 1994 |
| PubMed ID: 7959758 |
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| Hendershot LM, Valentine VA, Lee AS, Morris SW, Shapiro DN, Localization of the gene encoding human BiP/GRP78, the endoplasmic reticulum cognate of the HSP70 family, to chromosome 9q34. Genomics20:281-4 1994 |
| PubMed ID: 8020977 |
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| Maraia RJ, Sasaki-Tozawa N, Driscoll CT, Green ED, Darlington GJ, The human Y4 small cytoplasmic RNA gene is controlled by upstream elements and resides on chromosome 7 with all other hY scRNA genes Nucleic acids research22:3045-52 1994 |
| PubMed ID: 7520568 |
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| Nagaoka H, Ozawa K, Matsuda F, Hayashida H, Matsumura R, Haino M, Shin EK, Fukita Y, Imai T, Anand R, et al, Recent translocation of variable and diversity segments of the human immunoglobulin heavy chain from chromosome 14 to chromosomes 15 and 16. Genomics22:189-97 1994 |
| PubMed ID: 7959766 |
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| Weiss J, Rubinfeld B, Polakis PG, McCormick F, Cavenee WK, Arden KC, The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-->p35. Cytogenet Cell Genet66:18-21 1994 |
| PubMed ID: 8275700 |
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| Chong SS, Kristjansson K, Zoghbi HY, Hughes MR, Molecular cloning of the cDNA encoding a human renal sodium phosphate transport protein and its assignment to chromosome 6p21.3-p23. Genomics18:355-359 1993 |
| PubMed ID: 8288239 |
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| Clark CC, Cohen I, Eichstetter I, Cannizzaro LA, McPherson JD, Wasmuth JJ, Iozzo RV, Molecular cloning of the human proto-oncogene Wnt-5A and mapping of the gene (WNT5A) to chromosome 3p14-p21. Genomics18:249-60 1993 |
| PubMed ID: 8288227 |
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| Drwinga HL, Toji LH, Kim CH, Greene AE, Mulivor RA, NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2. Genomics16:311-4 1993 |
| PubMed ID: 8314568 |
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| Figlewicz DA, Delattre O, Guellaen G, Krizus A, Thomas G, Zucman J, Rouleau GA, Mapping of human gamma-glutamyl transpeptidase genes on chromosome 22 and other human autosomes. Genomics17:299-305 1993 |
| PubMed ID: 8104871 |
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| Howe JR, Lairmore TC, Veile R, Dou S, Wells SA Jr, Donis-Keller H, Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping. Hum Genet91:199-204 1993 |
| PubMed ID: 8478002 |
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| Maraia RJ, Driscoll CT, Bilyeu T, Hsu K, Darlington GJ, Multiple dispersed loci produce small cytoplasmic Alu RNA. Mol Cell Biol13:4233-41 1993 |
| PubMed ID: 7686619 |
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| Washington SS, Bowcock AM, Gerken S, Matsunami N, Lesh D, Osborne-Lawrence SL, Cowell J, Ledbetter DH, White RL, Chakravarti A, A somatic cell hybrid map of human chromosome 13. Genomics18:486-95 1993 |
| PubMed ID: 8307557 |
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| Aplan PD, Raimondi SC, Kirsch IR, Disruption of the SCL gene by a t(1;3) translocation in a patient with T cell acute lymphoblastic leukemia. J Exp Med176:1303-10 1992 |
| PubMed ID: 1402676 |
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| Kishimoto J, Spurr N, Liao M, Lizhi L, Emson P, Xu W, Localization of brain nitric oxide synthase (NOS) to human chromosome 12 [published erratum appears in Genomics 1993 Feb;15(2):465] Genomics14:802-4 1992 |
| PubMed ID: 1385308 |
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| Morris SW, Nelson N, Valentine MB, Shapiro DN, Look AT, Kozlosky CJ, Beckmann MP, Cerretti DP, Assignment of the genes encoding human interleukin-8 receptor types 1 and 2 and an interleukin-8 receptor pseudogene to chromosome 2q35. Genomics14:685-91 1992 |
| PubMed ID: 1427896 |
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| Youngman S, Bates GP, Williams S, McClatchey AI, Baxendale S, Sedlacek Z, Altherr M, Wasmuth JJ, MacDonald ME, Gusella JF, et al, The telomeric 60 kb of chromosome arm 4p is homologous to telomeric regions on 13p, 15p, 21p, and 22p. Genomics14:350-6 1992 |
| PubMed ID: 1427851 |
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| Mares A Jr, Ledbetter SA, Ledbetter DH, Roberts R, Hejtmancik JF, Isolation of a human chromosome 14-only somatic cell hybrid: analysis using Alu and LINE-based PCR. Genomics11:215-8 1991 |
| PubMed ID: 1765380 |
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| Ledbetter SA, Garcia-Heras J, Ledbetter DH, "PCR-karyotype" of human chromosomes in somatic cell hybrids. Genomics8:614-22 1990 |
| PubMed ID: 2276735 |
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| Lugo TG, Handelin B, Killary AM, Housman DE, Fournier RE, Isolation of microcell hybrid clones containing retroviral vector insertions into specific human chromosomes. Mol Cell Biol7:2814-20 1987 |
| PubMed ID: 2823107 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Geneticin 0.25 mg/ml |
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