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I91S-05 Fibroblast from Skin, Skin

Description:

IMR-91 - NORMAL HUMAN FETAL FIBROBLAST

Affected:

No

Sex:

Male

Age:

12 FW (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIA Aging Cell Culture Repository
Subcollection IMR90/IMR91 Cell Cultures
Specially Characterized Fibroblasts
Biopsy Source Skin
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Sample Source Fibroblast from Skin, Skin
Race White
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks The IMR-91 skin-derived line was initiated on 2/24/76 from explants of minced skin tissue from an approximately 12 week fetus. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. The HLA type is A2, A11, B18 and B5. G6PD is isoenzyme type B and 6PGD is isoenzyme type A. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Characterizations

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PDL at Freeze 10
Passage Frozen 10
 

Phenotypic Data

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Remarks The IMR-91 skin-derived line was initiated on 2/24/76 from explants of minced skin tissue from an approximately 12 week fetus. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. The HLA type is A2, A11, B18 and B5. G6PD is isoenzyme type B and 6PGD is isoenzyme type A. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Publications

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Nichols WW, Cristofalo VJ, Toji LH, Greene AE, Aronson MM, Dwight S, Charpentier R, Hoffman E, Characterization of a new human diploid cell line--IMR-91. In Vitro19:797-804 1983
PubMed ID: 6629383
 
Brown WT, Ford JP, Gershey EL, Variation of DNA repair capacity in progenia cells unrelated to growth conditions. Biochem Biophys Res Commun97:347-53 1980
PubMed ID: 7458939

External Links

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dbSNP dbSNP ID: 16899

Culture Protocols

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Cumulative PDL at Freeze 20
Passage Frozen 10
Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA
Supplement -
Pricing
Commercial:
$257.00USD
Academic &
Non-profit:
$103.00USD
NIA Grantees:
$47.00USD
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