AG02102
Fibroblast from Endometrial, Uterus
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Uterus
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Cell Type
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Fibroblast
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Tissue Type
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Endometrial
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Endometrial, Uterus
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The culture was initiated using explants of endometrial tissue obtained from a uterine curettage procedure performed 1/10/77. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal diploid female. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Noumoff J, Haydock SW, Sachdeva R, Heyner S, Pritchard ML, Characteristics of cell lines derived from normal and malignant endometrial tissue. Gynecol Oncol27:141-9 1987 |
| PubMed ID: 2436984 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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