AG04061
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY/46,XY,t(8;18)(8pter>8q11::18q11> 18qter;18pter>18q11::8q11>8qter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
53 |
| PDL at Freeze |
9 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XY/46,XY,t(8;18)(8pter>8q11::18q11>18qter;18pter>18q11:: 8q11>8qter); balanced; 80%/20%. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Thomas R, Moloney EB, Macbain ZK, Hallett PJ, Isacson O, Fibroblasts from idiopathic Parkinson's disease exhibit deficiency of lysosomal glucocerebrosidase activity associated with reduced levels of the trafficking receptor LIMP2 Molecular brain14:16 2020 |
| PubMed ID: 33468204 |
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| Reed, TGF-beta 1 induces the expression of type 1 collagen and SPARC, and enhances contraction of collagen gels, by fibroblasts from young and aged donors. J Cell Biol158:169 (1994):16 1994 |
| PubMed ID: 33468204 |
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| McCoy KR, Mullins RD, Newcomb TG, Ng GM, Pavlinkova G, Polinsky RJ, Nee LE, Sisken JE, Serum- and bradykinin-induced calcium transients in familial Alzheimer's fibroblasts. Neurobiol Aging14:447-55 1993 |
| PubMed ID: 8247227 |
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| Schneider EL, Monticone R, Smith J, Braunschweiger K, Roberts T, Skin fibroblast cultures derived from members of the Baltimore longitudinal study: a new resource for studies of cellular aging. Cytogenet Cell Genet31:40-6 1981 |
| PubMed ID: 7307581 |
| PDL at Senescence |
53 |
| Cumulative PDL at Freeze |
9 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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