AG04153
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Family Member
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3
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Relation to Proband
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father
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY,der(9)(9qter>9p23::7q11>7qter)[3]/46,XY,der(13)(13qter>13p13::7q11>7qter)[2]/46,XY,add(13)(?::p13>qter)[2]/46,XY[43]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
10 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The karyotype is showing greater instability with the in vitro aging of each succeeding expansion for stock. AG04153B displays less stability than AG04153. Analysis of AG04153 showed random chromosome loss in 6% of the cells; no random chromosome gains and random chromosomal aberrations in 4% of the cells. One of these was an add(13)(p13), but the additional material is noticeably different. Culture grows slowly. Donor is the father of AG04438 and AG05244. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Schneider EL, Monticone R, Smith J, Braunschweiger K, Roberts T, Skin fibroblast cultures derived from members of the Baltimore longitudinal study: a new resource for studies of cellular aging. Cytogenet Cell Genet31:40-6 1981 |
| PubMed ID: 7307581 |
| Cumulative PDL at Freeze |
10 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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