AG04351
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Family Member
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6
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Relation to Proband
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nephew
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
42 |
| PDL at Freeze |
11 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like and the karyotype is 46,XY; diploid male. Donor is the nephew of AG05839A and the cousin of AG06285A and AG09854. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Chen ZS1,2, Li L1,2, Peng S1,2, Chen FM3, Zhang Q1,2, An Y1,2, Lin X2, Li W2, Koon AC1,2, Chan TF2,3,4,5, Lau KF2,3,4, Ngo JCK2,3, Wong WT3, Kwan KM3,6,7, Chan HYE8,2,3,4,5., Planar cell polarity gene Fuz triggers apoptosis in neurodegenerative disease models EMBO Reports: 2018 |
| PubMed ID: 30026307 |
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| Clemmons DR, Shaw DS, Variables controlling somatomedin production by cultured human fibroblasts. J Cell Physiol115:137-42 1983 |
| PubMed ID: 6601662 |
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| Schneider EL, Monticone R, Smith J, Braunschweiger K, Roberts T, Skin fibroblast cultures derived from members of the Baltimore longitudinal study: a new resource for studies of cellular aging. Cytogenet Cell Genet31:40-6 1981 |
| PubMed ID: 7307581 |
| PDL at Senescence |
42 |
| Cumulative PDL at Freeze |
11 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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