AG04677
Epithelial from Eye
Description:
ORYCTOLAGUS
ORDER: LAGOMORPHA
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Animals |
|
Biopsy Source
|
Eye
|
|
Cell Type
|
Epithelial
|
|
Tissue Type
|
Lens
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Epithelial from Eye
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Oryctolagus cuniculus
|
|
Common Name
|
rabbit
|
|
Remarks
|
|
| PDL at Freeze |
39 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Genbank |
COX1 |
| Remarks |
The culture was initiated on 9/1/80 from the ocular lens capsule of a New Zealand White rabbit. Cell morphology is epithelial-like. The karyotype is 44,XX; normal diploid female. The culture can be carried for an additional 40 passages after recovery from liquid nitrogen storage. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Hofmann W, Schubert D, LaBonte J, Munson L, Gibson S, Scammell J, Ferrigno P, Sodroski J, Species-specific, postentry barriers to primate immunodeficiency virus infection. J Virol73:10020-8 1999 |
| PubMed ID: 10559316 |
| |
| Megraud F, Gamon LB, McGarrity GJ, Characterization of Spiroplasma mirum (suckling mouse cataract agent) in a rabbit lens cell culture. Infect Immun42:1168-75 1983 |
| PubMed ID: 6642663 |
| |
| Reddan JR, Friedman TB, Mostafapour MK, Bondy RL, Sutherland SH, McGee SJ, Goldenberg EM, Donor age influences the growth of rabbit lens epithelial cells in vitro. Vision Res21:11-23 1981 |
| PubMed ID: 7269278 |
| Cumulative PDL at Freeze |
39 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|