AG05055

AG05055 Epithelial from Eye

Description:

MUS
ORDER: RODENTIA

Affected:

No Data

Sex:

Male

Age:

1 DA (At Sampling)

Overview

Repository

NIA Aging Cell Culture Repository

Subcollection

Animals

Biopsy Source

Eye

Cell Type

Epithelial

Tissue Type

Lens

Transformant

Untransformed

Sample Source

Epithelial from Eye

Relation to Proband

proband

Confirmation

Clinical summary/Case history

ISCN

Heteroploid; 100% chromosomally abnormal counts range from 54-100

Species

Mus musculus

Common Name

house mouse

Remarks

Mouse strain CD-1 (Charles River outbred line). The culture was initiated on 1/29/80 using cells released by trypsin treatment of a lens capsule which previously had been maintained in organ culture for 5 days. The cells grow abnormally in culture and the cell morphology is epitheliallike. This line is heteroploid with all cells examined being chromosomally abnormal.

Characterizations

PDL at Freeze

IDENTIFICATION OF SPECIES OF ORIGIN

Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis

Genbank

COX1

Phenotypic Data

Remarks

External Links

dbSNP

dbSNP ID: 15425

Culture Protocols

Cumulative PDL at Freeze

Split Ratio

1:5

Temperature

37 C

Percent CO2

Percent O2

AMBIENT

Medium

Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent

Serum

20% fetal bovine serum Not inactivated

Substrate

None specified

Subcultivation Method

trypsin-EDTA

Supplement

Pricing

Commercial:

$257.00USD

Academic &
Non-profit:

$103.00USD

NIA Grantees:

$47.00USD