AG05120
Fibroblast from Umbilical Cord, Umbilical cord
Description:
TRISOMY 21
NIA AGING CELL REPOSITORY DNA PANEL - DOWN SYNDROME
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Chromosome Abnormalities
Heritable Diseases |
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Biopsy Source
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Umbilical cord
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Cell Type
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Fibroblast
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Tissue Type
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Umbilical Cord
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Umbilical Cord, Umbilical cord
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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47,XX,+21
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
9 |
| Passage Frozen |
8 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
This culture was derived from minced umbilical cord tissue obtained from a therapeutically aborted fetus of 22 weeks gestational age. The karyotype is 47,XX,+21. The cell morphology is fibroblast-like. Culture was frozen at PDL 3. An amniotic fluid fibroblast culture established from same donor is AG05121. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Kinde I, Papadopoulos N, Kinzler KW, Vogelstein B, FAST-SeqS: a simple and efficient method for the detection of aneuploidy by massively parallel sequencing PloS one7:e41162 2012 |
| PubMed ID: 22815955 |
| Cumulative PDL at Freeze |
9 |
| Passage Frozen |
8 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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