AG05352
Fibroblast from Skin, Thigh
Description:
ATELES GEOFFROYI
ORDER: PRIMATES
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Nonhuman Primates Animals |
Biopsy Source
|
Thigh
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Thigh
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
34,XY
|
Species
|
Ateles geoffroyi
|
Common Name
|
black-handed spider monkey
|
Remarks
|
|
PDL at Freeze |
16 |
Passage Frozen |
10 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
|
Genbank |
COX1 |
Remarks |
Black-handed spider monkey. |
Diehl WE, Stansell E, Kaiser SM, Emerman M, Hunter E, Identification of Post-entry Restrictions to Mason-Pfizer Monkey Virus Infection in New World Monkey Cells Journal of virology82(22):11140-50 2008 |
PubMed ID: 18799582 |
|
Sawyer SL, Wu LI, Emerman M, Malik HS, Positive selection of primate TRIM5alpha identifies a critical species-specific retroviral restriction domain. Proc Natl Acad Sci U S A102(8):2832-7 2005 |
PubMed ID: 15689398 |
|
Song B, Gold B, O'Huigin C, Javanbakht H, Li X, Stremlau M, Winkler C, Dean M, Sodroski J, The B302(SPRY) domain of the retroviral restriction factor TRIM5alpha exhibits lineage-specific length and sequence variation in primates Journal of virology79:6111-21 2005 |
PubMed ID: 15857996 |
|
Qian Y, Jin L, Su B, Construction and characterization of bacterial artificial chromosome library of black-handed spider monkey (Ateles geoffroyi) Genome / National Research Council Canada = GĂ©nome / Conseil national de recherches Canada47:239-45 2004 |
PubMed ID: 15060576 |
|
Ejima Y, Yang L, Trans mobilization of genomic DNA as a mechanism for retrotransposon-mediated
exon shuffling. Hum Mol Genet12(11):1321-8 2003 |
PubMed ID: 12761047 |
|
Steinert S, White DM, Zou Y, Shay JW, Wright WE, Telomere biology and cellular aging in nonhuman primate cells. Exp Cell Res272(2):146-52 2002 |
PubMed ID: 11777339 |
|
Schempp W, Weber B, Muller G, Mammalian sex-chromosome evolution: a conserved homoeologous segment on the X and Y chromosomes in primates. Cytogenet Cell Genet50:201-5 1989 |
PubMed ID: 2805817 |
Cumulative PDL at Freeze |
16 |
Passage Frozen |
10 |
Split Ratio |
1:8 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|