AG06253
Fibroblast from Skin, Arm
Description:
MACACA MULATTA
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Nonhuman Primates
Animals |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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42,XX
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Species
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Macaca mulatta
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Common Name
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rhesus monkey
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Remarks
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| PDL at Senescence |
13 |
| PDL at Freeze |
4 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Indian strain of rhesus macaque; the age of the donor is estimated. The donor was acquired as an adult at the Delta Regional Primate Center. The age given is the sum of the years at the center plus 5 years (the minimum age for an adult rhesus monkey). The skin biopsy was obtained on 1/11/83, ante-mortem, from the inner aspect of the upper arm. |
| PDL at Senescence |
13 |
| Cumulative PDL at Freeze |
4 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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