AG06254
Fibroblast from Skin, Arm
Description:
ERYTHROCEBUS PATAS
ORDER: PRIMATES
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Nonhuman Primates
Animals |
|
Biopsy Source
|
Arm
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Arm
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
54,XX
|
|
Species
|
Erythrocebus patas
|
|
Common Name
|
patas monkey (hussar or red guenon)
|
|
Remarks
|
|
| PDL at Senescence |
45 |
| PDL at Freeze |
4 |
| Passage Frozen |
2 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Genbank |
COX1 |
| Remarks |
The biopsy was taken ante-mortem on 1/11/83 from skin of the inner aspect of the upper arm. The animal's birthdate is not known and her age is estimated. |
| PDL at Senescence |
45 |
| Cumulative PDL at Freeze |
4 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|