AG06814-J
Fibroblast from Lung, Lung
Description:
WI-38 - NORMAL HUMAN FETAL LUNG FIBROBLAST
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Specially Characterized Fibroblasts |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Race
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White
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Family Member
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2
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
50 |
| Passage Frozen |
16 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The WI-38 cell line was developed in July 1962 from lung tissue taken from a therapeutically aborted fetus of about 3 months gestational age. Cells released by trypsin digestion of the lung tissue were used for the primary culture. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal diploid female. A maximum lifespan of 50 population doublings for this culture was obtained at the Repository. A thymidine labelling index of 86% was obtained after recovery. G6PD is isoenzyme type B. This culture of WI-38 is an expansion from passage 9 frozen cells obtained from the submitter. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
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| PubMed ID: 37083495 |
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| PubMed ID: 36528129 |
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| PubMed ID: 36755096 |
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| PubMed ID: 33159050 |
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| PubMed ID: 32631951 |
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| PubMed ID: 31658304 |
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| PubMed ID: 29757379 |
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| PubMed ID: 30675059 |
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| Anderton RS, Meloni BP, Mastaglia FL, Greene WK, Boulos S, Survival of motor neuron protein over-expression prevents calpain-mediated cleavage and activation of procaspase-3 in differentiated human SH-SY5Y cells Neuroscience181:226-33 2010 |
| PubMed ID: 21333717 |
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| Marasa BS, Srikantan S, Martindale JL, Kim MM, Lee EK, Gorospe M, Abdelmohsen K, MicroRNA profiling in human diploid fibroblasts uncovers miR-519 role in replicative senescence Aging2:333-43 2010 |
| PubMed ID: 20606251 |
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| Li B, Jog SP, Reddy S, Comai L, WRN controls formation of extrachromosomal telomeric circles and is required for TRF2DeltaB-mediated telomere shortening Molecular and cellular biology28:1892-904 2008 |
| PubMed ID: 18212065 |
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| Dauth I, Krüger J, Hofmann TG, Homeodomain-interacting protein kinase 2 is the ionizing radiation-activated p53 serine 46 kinase and is regulated by ATM Cancer research67:2274-9 2007 |
| PubMed ID: 17332358 |
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| Tresini M, Lorenzini A, Torres C, Cristofalo VJ, Modulation of replicative senescence of diploid human cells by nuclear ERK signaling J Biol Chem282(6):4136-51 2007 |
| PubMed ID: 17145763 |
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| Singh NK, Singh NN, Androphy EJ, Singh RN, Splicing of a critical exon of human Survival Motor Neuron is regulated by a unique silencer element located in the last intron Molecular and cellular biology26:1333-46 2006 |
| PubMed ID: 16449646 |
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| Gosslau A, Chen M, Ho CT, Chen KY, A methoxy derivative of resveratrol analogue selectively induced activation of the mitochondrial apoptotic pathway in transformed fibroblasts Br J Cancer92(3):513-21 2005 |
| PubMed ID: 15668717 |
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| Michishita E, Park JY, Burneskis JM, Barrett JC, Horikawa I, Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins Molecular biology of the cell16:4623-35 2005 |
| PubMed ID: 16079181 |
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| Wang W, Martindale JL, Yang X, Chrest FJ, Gorospe M., Increased stability of the p16 mRNA with replicative senescence. EMBO Rep6(2):158-64 2005 |
| PubMed ID: 15678155 |
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| Kahl CR, Means AR, Regulation of cyclin D1/Cdk4 complexes by calcium/calmodulin-dependent protein kinase I. J Biol Chem279(15):15411-9 2004 |
| PubMed ID: 14754892 |
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| Kahl CR, Means AR, Calcineurin regulates cyclin D1 accumulation in growth-stimulated fibroblasts. Mol Biol Cell15(4):1833-42 2004 |
| PubMed ID: 14767060 |
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| Marchesini N, Osta W, Bielawski J, Luberto C, Obeid LM, Hannun YA, Role for mammalian neutral sphingomyelinase 2 in confluence-induced growth arrest of MCF7 cells. J Biol Chem279(24):25101-11 2004 |
| PubMed ID: 15051724 |
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| Sedelnikova OA, Horikawa I, Zimonjic DB, Popescu NC, Bonner WM, Barrett JC, Senescing human cells and ageing mice accumulate DNA lesions with unrepairable double-strand breaks. Nat Cell Biol6(2):168-70 2004 |
| PubMed ID: 14755273 |
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| Silverman J, Takai H, Buonomo SB, Eisenhaber F, de Lange T, Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint. Genes Dev18(17):2108-19 2004 |
| PubMed ID: 15342490 |
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| Von Kobbe C, May A, Grandori C, Bohr VA, Werner syndrome cells escape hydrogen peroxide-induced cell proliferation arrest. FASEB J18(15):1970-2 2004 |
| PubMed ID: 15459124 |
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| Xin H, Pereira-Smith OM, Choubey D, Role of IFI 16 in cellular senescence of human fibroblasts. Oncogene23(37):6209-17 2004 |
| PubMed ID: 15208661 |
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| Baird DM, Rowson J, Wynford-Thomas D, Kipling D, Extensive allelic variation and ultrashort telomeres in senescent human cells. Nat Genet33(2):203-7 2003 |
| PubMed ID: 12539050 |
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| Li GZ, Eller MS, Firoozabadi R, Gilchrest BA, Evidence that exposure of the telomere 3' overhang sequence induces senescence Proceedings of the National Academy of Sciences of the United States of America100:527-31 2003 |
| PubMed ID: 12515865 |
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| Schawalder J, Paric E, Neff NF, Telomere and ribosomal DNA repeats are chromosomal targets of the bloom syndrome DNA helicase. BMC Cell Biol4:15:527-31 2003 |
| PubMed ID: 14577841 |
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| Ding W, Gao S, Scott RE, Senescence represses the nuclear localization of the serum response factor and differentiation regulates its nuclear localization with lineage specificity. J Cell Sci114(Pt 5):1011-8 2001 |
| PubMed ID: 11181183 |
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| Hande MP, Balajee AS, Tchirkov A, Wynshaw-Boris A, Lansdorp PM, Extra-chromosomal telomeric DNA in cells from Atm(-/-) mice and patients with ataxia-telangiectasia. Hum Mol Genet10(5):519-28 2001 |
| PubMed ID: 11181576 |
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| PubMed ID: 11103814 |
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| Luberto C, Hannun YA, Sphingomyelin synthase, a potential regulator of intracellular levels of ceramide and diacylglycerol during SV40 transformation. Does sphingomyelin synthase account for the putative phosphatidylcholine-specific phospholipase C? J Biol Chem273:14550-9 1998 |
| PubMed ID: 9603970 |
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| Gamard CJ, Dbaibo GS, Liu B, Obeid LM, Hannun YA, Selective involvement of ceramide in cytokine-induced apoptosis. Ceramide inhibits phorbol ester activation of nuclear factor kappaB. J Biol Chem272(26):16474-81 1997 |
| PubMed ID: 9195956 |
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| PubMed ID: 7534307 |
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| PubMed ID: 7568133 |
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| PubMed ID: 8530509 |
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| PubMed ID: 8174635 |
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| PubMed ID: 8206919 |
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| PubMed ID: 2164149 |
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| PubMed ID: 2739467 |
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| Giordano T, Foster DN, Identification of a highly abundant cDNA isolated from senescent WI-38 cells. Exp Cell Res185:399-406 1989 |
| PubMed ID: 2480908 |
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| Giordano T, Kleinsek D, Foster DN, Increase in abundance of a transcript hybridizing to elongation factor I alpha during cellular senescence and quiescence. Exp Gerontol24:501-13 1989 |
| PubMed ID: 2483690 |
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| PubMed ID: 2474570 |
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| PubMed ID: 4007059 |
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| PubMed ID: 4007059 |
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| Hayflick, The serial cultivation of human diploid cell strains. Exp Cell Res25:585 (1961):342-8 1961 |
| PubMed ID: 4007059 |
| PDL at Senescence |
50 |
| Cumulative PDL at Freeze |
44 |
| Passage Frozen |
16 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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