AG07095
Fibroblast from Skin, Foreskin
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Foreskin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Foreskin
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Race
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Black/African American
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5.47 |
| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
The culture was initiated on 10/14/83 from explants of minced foreskin removed ante-mortem 4 hours earlier. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Catarinella G, Nicoletti C, Bracaglia A, Procopio P, Salvatori I, Taggi M, Valle C, Ferri A, Canipari R, Puri PL, Latella L, SerpinE1 drives a cell-autonomous pathogenic signaling in Hutchinson-Gilford progeria syndrome Cell death & disease13:737 2022 |
| PubMed ID: 36028501 |
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| Hartmann C, Herling L, Hartmann A, Köckritz V, Fuellen G, Walter M, Hermann A, Systematic estimation of biological age of Frontiers in aging4:1129107 2022 |
| PubMed ID: 36873743 |
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| Whalen S, Inoue F, Ryu H, Fair T, Markenscoff-Papadimitriou E, Keough K, Kircher M, Martin B, Alvarado B, Elor O, Laboy Cintron D, Williams A, Hassan Samee MA, Thomas S, Krencik R, Ullian EM, Kriegstein A, Rubenstein JL, Shendure J, Pollen AA, Ahituv N, Pollard KS, Machine learning dissection of human accelerated regions in primate neurodevelopment Neuron111:857-873.e8 2022 |
| PubMed ID: 36640767 |
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| Frankel D, Delecourt V, Novoa-Del-Toro EM, Robin JD, Airault C, Bartoli C, Carabalona A, Perrin S, Mazaleyrat K, De Sandre-Giovannoli A, Magdinier F, Baudot A, Lévy N, Kaspi E, Roll P, miR-376a-3p and miR-376b-3p overexpression in Hutchinson-Gilford progeria fibroblasts inhibits cell proliferation and induces premature senescence iScience25:103757 2021 |
| PubMed ID: 35118365 |
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| Murie M, Peng Y, Rigby MJ, Dieterich IA, Farrugia MA, Endresen A, Bhattacharyya A, Puglielli L, ATase inhibition rescues age-associated proteotoxicity of the secretory pathway Communications biology5:173 2021 |
| PubMed ID: 35217767 |
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| D'Acunzo P, Pérez-González R, Kim Y, Hargash T, Miller C, Alldred MJ, Erdjument-Bromage H, Penikalapati SC, Pawlik M, Saito M, Saito M, Ginsberg SD, Neubert TA, Goulbourne CN, Levy E, Mitovesicles are a novel population of extracellular vesicles of mitochondrial origin altered in Down syndrome Science advances7:173 2020 |
| PubMed ID: 33579698 |
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| Marentette JO, Anderson CC, Prutton KM, Jennings EQ, Rauniyar AK, Galligan JJ, Roede JR, Trisomy 21 impairs PGE2 production in dermal fibroblasts Prostaglandins & other lipid mediators153:106524 2020 |
| PubMed ID: 33418267 |
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| Mukherjee S, Gagne AL, Maguire JA, Jobaliya CD, Mills JA, Gadue P, French DL, Generation of human control iPSC line CHOPi004-A from juvenile foreskin fibroblast cells Stem cell research49:102084 2020 |
| PubMed ID: 33202304 |
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| Bordi M, Darji S, Sato Y, Mellén M, Berg MJ, Kumar A, Jiang Y, Nixon RA, mTOR hyperactivation in Down Syndrome underlies deficits in autophagy induction, autophagosome formation, and mitophagy Cell death & disease10:563 2019 |
| PubMed ID: 31332166 |
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| Desgrouas C, Varlet AA, Dutour A, Galant D, Merono F, Bonello-Palot N, Bourgeois P, Lasbleiz A, Petitjean C, Ancel P, Levy N, Badens C, Gaborit B, Unraveling Cells9:563 2019 |
| PubMed ID: 32012908 |
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| Jiang Y, Sato Y, Im E, Berg M, Bordi M, Darji S, Kumar A, Mohan PS, Bandyopadhyay U, Diaz A, Cuervo AM, Nixon RA, Lysosomal Dysfunction in Down Syndrome Is APP-Dependent and Mediated by APP-ßCTF (C99) The Journal of neuroscience : the official journal of the Society for Neuroscience39:5255-5268 2019 |
| PubMed ID: 31043483 |
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| Pérez-González R, Gauthier SA, Sharma A, Miller C, Pawlik M, Kaur G, Kim Y, Levy E, A pleiotropic role for exosomes loaded with the amyloid ß precursor protein carboxyl-terminal fragments in the brain of Down syndrome patients Neurobiology of aging84:26-32 2019 |
| PubMed ID: 31479861 |
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| Sebestyén E, Marullo F, Lucini F, Petrini C, Bianchi A, Valsoni S, Olivieri I, Antonelli L, Gregoretti F, Oliva G, Ferrari F, Lanzuolo C, SAMMY-seq reveals early alteration of heterochromatin and deregulation of bivalent genes in Hutchinson-Gilford Progeria Syndrome Nature communications11:6274 2019 |
| PubMed ID: 33293552 |
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| Yang SJ, Lipnick SL, Makhortova NR, Venugopalan S, Fan M, Armstrong Z, Schlaeger TM, Deng L, Chung WK, O'Callaghan L, Geraschenko A, Whye D, Berndl M, Hazard J, Williams B, Narayanaswamy A, Ando DM, Nelson P, Rubin LL, Applying Deep Neural Network Analysis to High-Content Image-Based Assays SLAS discovery : advancing life sciences R & D24:829-841 2019 |
| PubMed ID: 31284814 |
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| Wang L1, Smith J2, Breton C1, Clark P1, Zhang J1, Ying L1, Che Y1, Lape J2, Bell P1, Calcedo R1, Buza EL1, Saveliev A1, Bartsevich VV2, He Z1, White J1, Li M3, Jantz D2, Wilson JM1., Meganuclease targeting of PCSK9 in macaque liver leads to stable reduction in serum cholesterol Nat Biotechnol36:717-725 2018 |
| PubMed ID: 29985478 |
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| Liu Y, Xue Y, Ridley S, Zhang D, Rezvani K, Fu XD, Wang H, Direct reprogramming of Huntington's disease patient fibroblasts into neuron-like cells leads to abnormal neurite outgrowth, increased cell death, and aggregate formation PloS one9:e109621 2014 |
| PubMed ID: 25275533 |
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| Coffinier C, Hudon SE, Lee R, Farber EA, Nobumori C, Miner JH, Andres DA, Spielmann HP, Hrycyna CA, Fong LG, Young SG, A potent HIV protease inhibitor, darunavir, does not inhibit ZMPSTE24 or lead to an accumulation of farnesyl-prelamin A in cells The Journal of biological chemistry283:9797-804 2008 |
| PubMed ID: 18230615 |
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| Russanova VR, Hirai TH, Tchernov AV, Howard BH, Mapping development-related and age-related chromatin remodeling by a high throughput ChIP-HPLC approach The journals of gerontology Series A, Biological sciences and medical sciences59:1234-43 2005 |
| PubMed ID: 15699522 |
| Cumulative PDL at Freeze |
8.47 |
| Passage Frozen |
3 |
| Split Ratio |
1:9 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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