AG07430
Smooth muscle from Aorta, thoracic
Description:
CANIS
ORDER: CARNIVORA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Smooth muscle
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Smooth muscle from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Canis familiaris
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Common Name
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dog
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Remarks
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| PDL at Freeze |
20 |
| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
Beagle breed. The thoracic aorta was removed post-mortem on 3/15/84. Strips of the intimal and medial layers of the vessel were minced and treated with collagenase and elastase. Cells released by this treatment were used for the primary culture. Cell morphology is fibroblast-like. The karyotype is 78,XX; normal diploid female. The culture tested positive for alpha-smooth muscle actin and negative for the endothelial cell-specific von Willebrand factor (immunofluorescence assays). A skin fibroblast culture from this dog is AG07429. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Cumulative PDL at Freeze |
20 |
| Passage Frozen |
4 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 medium/Medium 199, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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